PXD069475

PXD069475 is an original dataset announced via ProteomeXchange.

Title	Plasma proteomics analysis in Atrial Septal Defect: Biomarker Discovery Utilizing WGCNA and Machine Learning Techniques
Description	Atrial septal defect (ASD) poses challenges for diagnosis and risk stratification. The role of proteomic dysregulation remains underexplored. This study integrates plasma proteomics, co-expression networks, and machine learning to identify ASD diagnostic biomarkers. Methods: We characterized the plasma proteome of 82 ASD patients and 87 controls using 10x Blood + Data independent acquisition (DIA) mass spectrometry, quantifying 5,892 proteins. Weighted co-expression network analysis (WGCNA) identified phenotype-associated protein modules. Machine learning (logistic regression with feature selection) prioritized biomarkers, validated by enzyme-linked immunosorbent assay (ELISA). Results: 348 proteins were differentially expressed in ASD (130 up; 218 down). Functional enrichment revealed dysregulation in ribosomal subunits, extracellular matrix organization, and neutrophil extracellular trap formation. Integration of WGCNA and machine learning defined a biomarker signature: RAB8B, COPS8, FN3K, and RPL36. ELISA confirmed significant dysregulation in independent cohorts. Conclusion: We identified candidate protein biomarkers potentially disrupting cardiac septal morphogenesis. These findings advance understanding of ASD's molecular basis and highlight diagnostic targets, offering implications for improved screening and treatment strategies
HostingRepository	iProX
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_01:14:56.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoshan Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-15 01:14:38	ID requested
⏵ 1	2025-10-15 01:14:57	announced

Dataset with its publication pending

submitter keyword: Atrial septal defect, Proteomics, WGCNA, Machine learning, Biomarker discovery

Xiaoshan Zhang
contact affiliation	The Affiliated Hospital of Inner Mongolia Medical University
contact email	13947133133@163.com
lab head
Xiaoshan Zhang
contact affiliation	The Affiliated Hospital of Inner Mongolia Medical University
contact email	13947133133@163.com
dataset submitter

iProX dataset URI