PXD069439

PXD069439 is an original dataset announced via ProteomeXchange.

Title	Multi-dimensional native proteomics technology for protein complex identification and topology characterization (Mud-NatPIT)
Description	Here, we demonstrate a pilot development of multi-dimensional native proteomics technology for protein complex identification and topology characterization (Mud-NatPIT) in complex biological samples via coupling native size exclusion chromatography (SEC), online native capillary electrophoresis-mass spectrometry (nCE-MS), surface-induced dissociation (SID), and multi-level proteomics. The Mud-NatPIT enabled the detection of 124 unique native endogenous protein complexes with masses up to 1.5 MDa from a whole cell lysate, identified 46 unique protein complexes in a discovery mode, and provided topological information for 39 protein complexes. The Mud-NatPIT is broadly applicable for characterizing protein complexes in cells, tissues, and biological fluids.
HostingRepository	PRIDE
AnnounceDate	2026-08-04
AnnouncementXML	Submission_2026-08-03_20:06:22.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fei FANG
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive UHMR; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-14 04:49:47	ID requested
⏵ 1	2026-08-03 20:06:23	announced

10.1021/ACS.ANALCHEM.6C01693;

submitter keyword: capillary electrophoresis-mass spectrometry, protein complex identification,native proteomics, topology characterization, surface-induced dissociation

Liangliang Sun
contact affiliation	Michigan State University
contact email	lsun@chemistry.msu.edu
lab head
Fei FANG
contact affiliation	Michigan State University
contact email	fiona.dicp@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD069439

PRIDE project URI

• PRIDE
  1. PXD069439
     1. Label: PRIDE project
     2. Name: Multi-dimensional native proteomics technology for protein complex identification and topology characterization (Mud-NatPIT)