PXD069435

PXD069435 is an original dataset announced via ProteomeXchange.

Title	AutoGP 2: an improved fully automated high-throughput system for simultaneously enriching and separating intact glycopeptides
Description	An improved fully automated high-throughput system, named as AutoGP 2, for simultaneously enriching and separating intact glycopeptides has been developed in this study by introducing a second 10-port valve (valve 2) to the previously developed AutoGP system with only a 10-port valve (valve 1). Specifically, the addition of the second 10-port valve in the new AutoGP 2 system allows simultaneous enrichment and separation of intact glycopeptides, which significantly improve the sample analysis throughput as compared to previously developed AutoGP system. The optimal operating parameters of the AutoGP 2 were systematically evaluated, and the intra-day and inter-day reproducibility of this system were assessed through 24-h and 15-day long stress tests. The experimental evaluation has shown outstanding sensitivity and reproducibility of AutoGP 2. Over 560 site-specific glycans were identified from 1 μg peptide mixture with median CV of 10.8% in triplicate sample analyses. By coupling the AutoGP 2 with a high resolution Orbitrap mass spectrometer, the duty cycle of the instrument for a single sample analysis was increased from 55% to 80%. Specifically, 166 minutes were saved for every two-sample analyses in the AutoGP2 as compared with the previous AutoGP system, which increased the sample analysis throughput by 1.5 times. All the experimental results have consistently shown that the AutoGP 2 is well-suited for large cohort clinical glycoproteomic sample analysis and very effective in the sample amount limited glycoproteomic analysis.
HostingRepository	iProX
AnnounceDate	2025-10-14
AnnouncementXML	Submission_2025-10-14_01:47:54.196.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhonghan Hu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	complex glycosylation
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-14 01:47:39	ID requested
⏵ 1	2025-10-14 01:47:54	announced

Dataset with its publication pending

submitter keyword: Glycosylation, Automated enrichment, Intact glycopeptide, High-reproducibility, High-throughput

Keqi Tang
contact affiliation	Ningbo University
contact email	tangkeqi@nbu.edu.cn
lab head
Zhonghan Hu
contact affiliation	Ningbo University
contact email	huzh05@foxmail.com
dataset submitter

iProX dataset URI