⮝ Full datasets listing
PXD069384
PXD069384 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of secretomes from WM9/Hs294T melanoma cells resistant and non-resistant to BRAF/MEK and from their co-cultures with adipocytes |
| Description | Treatment based on BRAF/MEK kinase inhibitors is currently one of the most commonly used methods in melanoma therapy. Unfortunately, in the case of advanced melanoma, patients often develop resistance to treatment at an early stage of the treatment. A thorough understanding of the causes and mechanisms may contribute to the development of new, more effective treatments. One of the ways in which treatment-resistant cells can affect other cancer cells and the tumor microenvironment is through the factors they secrete. Therefore, this study aimed to examine the protein composition of the secretome of cells resistant to vemurafenib (BRAF inhibitor) and cobimetinib (MEK inhibitor) and compare it with the secretome of non-resistant cells. Proteomic analysis, followed by gene ontology (GO) analysis, identified many differences in resistant melanoma cells' secretomes compared to controls (non-resistant) in terms of cellular compartment, molecular function, biological processes, and reactome pathways. Proteins unique to the secretomes of resistant cells were associated with, among others, integrin binding and interactions, and the extracellular matrix organization. Other interesting groups of proteins, i.e., up- or downregulated in secretomes of resistant melanoma cells vs their non-resistant variants, were directly related to cancer progression and associated with cell adhesion, actin cytoskeleton organization, matrix proteolysis, and drug resistance. Proteins included in these groups, once secreted by resistant melanoma cells, can undoubtedly influence the surrounding microenvironment in a way that promotes the formation of a pro-tumor niche. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-10-27 |
| AnnouncementXML | Submission_2025-10-27_02:45:24.981.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Magda Surman |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation; Acetyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-13 01:53:49 | ID requested | |
| ⏵ 1 | 2025-10-27 02:45:25 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: melanoma, drug resistance, secretome, BRAF inhibitor, vemurafenib, cobimetinib, MEK inhibitor, DatasetType:Proteomics |
Contact List
| Aleksandra Simiczyjew | |
|---|---|
| contact affiliation | Department of Cell Pathology, Faculty of Biotechnology, University of Wroclaw |
| contact email | aleksandra.simiczyjew@uwr.edu.pl |
| lab head | |
| Magda Surman | |
| contact affiliation | Jagiellonian University |
| contact email | magdalena.surman@uj.edu.pl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099457/ |
