PXD069348 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Adenovirus E4ORF1 activates isoform-specific phosphatidylinositol 3-kinase signaling |
| Description | The human adenovirus serotype 5 E4ORF1 (Ad5E4ORF1) protein promotes primary endothelial cell survival and angiocrine functions by hijacking the cellular phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway. However, the mechanism by which E4ORF1 activates PI3K in vascular cells remains largely unknown. Here we show that Ad5E4ORF1 recruits multiple host scaffold proteins including DLG1, which promotes AKT activation by both Ad5E4ORF1 and cell receptor agonists. Furthermore, Ad5E4ORF1 specifically engages human PI3K isoform p110a-p85b through multi-domain interactions exclusively with p110a. Importantly, various serotypes of E4ORF1 proteins differentially interact with p110a leading to graded AKT activation in endothelial cells. We propose that E4ORF1 specifically recognizes p110a-p85b and allosterically activates PI3K through multiple direct contacts with p110a, setting the stage of endothelial cell survival and homeostatic angiogenic functions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-05 |
| AnnouncementXML | Submission_2026-01-04_16:08:23.079.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | TuKiet Lam |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-11 01:00:31 | ID requested | |
| ⏵ 1 | 2026-01-04 16:08:24 | announced | |
Publication List
| Geng F, Kobayashi M, Lin Y, Gomez-Salinero JM, Romano D, Kanyo J, Geng J, Liu Y, Ginsberg M, Shieh JH, Chen K, Lam TT, Rafii A, Rabbany SY, Lis R, Rafii S, Adenovirus E4ORF1 activates isoform-specific phosphatidylinositol 3-kinase signaling in human endothelial cells. J Biol Chem, 302(1):110947(2026) [pubmed] |
| 10.1016/j.jbc.2025.110947; |
Keyword List
| submitter keyword: Adenovirus |
| AKT |
| angiogenesis |
| DLG1 |
| E4ORF1 |
| endothelium |
| phosphatidylinositol-3-kinase. |
Contact List
| TuKiet Lam |
|---|
| contact affiliation | Keck MS & Proteomics Resource Yale School of Medicine |
| contact email | tukiet.lam@yale.edu |
| lab head | |
| TuKiet Lam |
|---|
| contact affiliation | Yale University |
| contact email | tukiet.lam@yale.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD069348 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069348
- Label: PRIDE project
- Name: Adenovirus E4ORF1 activates isoform-specific phosphatidylinositol 3-kinase signaling
