PXD069322 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Optimised Sample Handling Minimises Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics |
| Description | Chemical proteomics approaches often yield low peptide recovery because they aim to enrich low-abundance proteins. Poor sample handling further reduces recovery through peptide adsorption to plastic surfaces and losses due to vacuum evaporation. We systematically mapped these losses across buffers, volumes, plastics and pH, then developed an Evotip-compatible handling protocol that minimises adsorption and removes the need for vacuum evaporation. Losses to plastic adsorption and vacuum evaporation were most apparent at low inputs (< 200 ng) and with larger volumes (> 20 µL) but were also dependent on acidification as well as the buffer and plastic used. The optimised workflow: direct acidification of peptides, frozen storage if needed, and direct loading onto Evotips, resulted in up to ~100-fold gains versus common drying practices. This optimised sample handling method enables high-throughput chemical proteomics by reducing manual handling steps and enables the characterisation of low abundance proteins previously lost during sample preparation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-23 |
| AnnouncementXML | Submission_2026-06-23_08:53:23.039.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jack Houghton |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-10 08:27:45 | ID requested | |
| ⏵ 1 | 2026-06-23 08:53:23 | announced | |
Publication List
Keyword List
| submitter keyword: evosep, optimisation,chemoproteomics |
Contact List
| Edward Tate |
|---|
| contact affiliation | Imperial College London |
| contact email | e.tate@imperial.ac.uk |
| lab head | |
| Jack Houghton |
|---|
| contact affiliation | Imperial College London |
| contact email | j.houghton@imperial.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD069322 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069322
- Label: PRIDE project
- Name: Optimised Sample Handling Minimises Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics
