PXD069282

PXD069282 is an original dataset announced via ProteomeXchange.

Title	Degron models: a toolbox for rapid in vivo depletion of essential proteins regulating mRNA metabolism
Description	Due to their essentiality, studying proteins involved in fundamental processes in vivo is challenging. PROTAC-based systems offer time-controlled protein depletion, but their characterization in vivo remains limited. Here, with an efficient direct zygote editing protocol, we generated degron-tag models (dTAG/FKBP or BromoTag) for seven genes involved in therapeutic and endogenous mRNA metabolism (Cnot1, Pan2, Tent5a, Tent4b, Dcp2, Rnasel, Tsg101). Degron tags occasionally caused phenotypes that were often mitigated by tag position or tagging system change. In cells, both approaches yielded rapid and sustained degradation. In mice, dTAG depletion was effective but varied by protein and administration route, whereas BromoTag showed no in vivo activity. We showcase the utility of these models through an analysis of CNOT1's roles in cell division, immunity, and poly(A) tail maintenance. We present a valuable toolbox for studying mRNA metabolism in mammalian models, while providing a benchmark for applying degron-tag models to study other biological processes.
HostingRepository	PRIDE
AnnounceDate	2026-02-26
AnnouncementXML	Submission_2026-02-26_03:30:01.610.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Linke
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-10 00:32:18	ID requested
⏵ 1	2026-02-26 03:30:02	announced

Dataset with its publication pending

submitter keyword: TMT,degron-tag, liver, mouse

Andrzej Dziembowski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw (IIMCB), 02109, Warsaw, Poland
contact email	adziembowski@iimcb.gov.pl
lab head
Vanessa Linke
contact affiliation	IIMCB (International Institute of Molecular and Cell Biology in Warsaw)
contact email	massspec.vanessa@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD069282

PRIDE project URI

• PRIDE
  1. PXD069282
     1. Label: PRIDE project
     2. Name: Degron models: a toolbox for rapid in vivo depletion of essential proteins regulating mRNA metabolism