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PXD069279

PXD069279 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProximity labeling-based proteomic analysis of the HEV ORF1-associated replication complex under EIF4H deficiency
DescriptionThis project explores the role of EIF4H in regulating the composition of the HEV ORF1-associated replication complex using proximity-dependent biotin labeling with TurboID. TurboID was inserted into a validated permissive site within the ORF1 coding region that allows insertion without disrupting ORF1 function. HEK293T wild-type and EIF4H-knockout cells expressing ORF1-TurboID were subjected to biotin labeling, streptavidin-based enrichment, and mass spectrometry analysis. Comparative proteomics identified EIF4H-dependent host factors associated with the HEV replication complex.
HostingRepositoryiProX
AnnounceDate2025-10-09
AnnouncementXMLSubmission_2025-10-10_00:28:48.330.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDong Lin
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Ascend
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-10-10 00:28:19ID requested
⏵ 12025-10-10 00:28:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: HEV, ORF1, proximity labeling
Contact List
Qiang Ding
contact affiliationTsinghua University
contact emailqding@tsinghua.edu.cn
lab head
Dong Lin
contact affiliationTsinghua University
contact emaildong-l19@mails.tsinghua.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI

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