PXD069257

PXD069257 is an original dataset announced via ProteomeXchange.

Title	Identifying overtraining biomarkers through proteomic analysis of extracellular vesicles derived from the central nervous system of male mice
Description	Overtraining, which results in central nervous system (CNS)-fatigue-related symptoms, lead to a long-term decline in performance. This study investigated CNS-derived EV protein content under overtraining conditions to identify potential biomarkers. Eight-week-old C57BL/6J mice were randomly divided into three groups: sedentary (SED, n=7), exercise control (EX, n=8), and overtraining (OT, n=9) groups. The OT underwent an 8-week downhill treadmill-based overtraining induction protocol. Exercise capacity was assessed using the incremental load, exhaustion, grip strength, and rotarod tests, while motor deficits, depression and anxiety were assessed using the nest building test. Proinflammatory cytokines were measured in blood plasm, skeletal muscle, and brain tissue. CNS-derived EVs were isolated using a two-step EV isolation protocol. Isolated EVs underwent proteomic analyses. Mice exhibited a significant decrease in aerobic exercise capacity, high-intensity exercise tolerance, and muscular strength. OT increased quadriceps IL-6 and hypothalamic IL-1β/TNFα compared to EX. Plasma IL-2 levels tended to be higher in OT than in EX. Proteomic analysis of CNS-derived EVs revealed a decrease in lipid metabolism-related proteins. Valosin-containing proteins and catalase, which are upregulated in organs under oxidative stress, were increased. Therefore, CNS-derived EV protein contents indicated CNS fatigue under overtraining conditions.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:41:20.407.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Heon Yi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-09 09:50:46	ID requested
⏵ 1	2025-11-09 16:41:21	announced

10.14814/phy2.70640;

Yi DH, Hwang ES, Ko KE, Kim TY, Cho YS, Yook KH, da Silva ASR, Moon HY, Identifying overtraining biomarkers through proteomic analysis of extracellular vesicles derived from the central nervous system of male mice. Physiol Rep, 13(21):e70640(2025) [pubmed]

submitter keyword: Biomarker, Extracellular vesicle, CNS-fatigue, Overtraining, Proteomic analysis

Hyo Youl Moon
contact affiliation	Exercise Biochemistry Lab of Department of Physical Education, Seoul National University
contact email	skyman19@snu.ac.kr
lab head
Dong Heon Yi
contact affiliation	Seoul National University
contact email	leoyi818@snu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069257
     1. Label: PRIDE project
     2. Name: Identifying overtraining biomarkers through proteomic analysis of extracellular vesicles derived from the central nervous system of male mice