PXD069213

PXD069213 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Anti-C-RAF IP-MS in MG-treated NCI-H226 cells
Description	NCI-H226 cells in 100 mm dishes were treated with 10 uM of compounds 22 or 23 or DMSO at equal volume (final [DMSO] = 0.02%) for 24 h in triplicates for each condition (n = 3). Soluble lysates proceeded to anti-endogenous CRAF IP-MS with anti-CRAF antibody (BD Biosciences 610152; incubated at 4C overnight) and protein A/G beads (Pierce 88802; incubated at RT for 1 h), and on-bead digestion with PreOmics sample cleanup kit. 200 ng of peptides were quantified and injected onto Orbitrap Eclipse with 60 min gradient using DIA.
HostingRepository	MassIVE
AnnounceDate	2025-10-08
AnnouncementXML	Submission_2025-10-08_12:22:29.103.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shu Luo
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Acetyl; Oxidation
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-08 12:15:12	ID requested
⏵ 1	2025-10-08 12:22:29	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Molecular glue, C-RAF, 14-3-3, SFN, DatasetType:Proteomics

submitter keyword: Molecular glue, C-RAF, 14-3-3, SFN, DatasetType:Proteomics

Contact List

Michelle Arkin
contact affiliation	UCSF
contact email	Michelle.Arkin@ucsf.edu
lab head
Shu Luo
contact affiliation	UCSF
contact email	Shu.Luo@ucsf.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099423/