PXD069198

PXD069198 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling of the SmSTRIPAK complex in Sordaria macrospora using the SCI1 subunit as bait to identify its SmSTRIPAK-dependent microenvironment
Description	In this study, we used the in vivo proximity labeling biotin identification (BioID; Roux et al., 2012) method to capture proteins of the microenvironment of the striatin interacting kinase and phosphatase complex of the filamentous fungus Sordaria macrospora (SmSTRIPAK). For that, the TurboID biotin ligase (Branon et al., 2018) was fused to the STRIPAK complex interactor 1 (sci1; SMAC_05559), a subunit of the SmSTRIPAK complex (Hollstein et al., 2022). We applied two control strains for relative quantification to reduce false positive hits. The first control expressed a free unfused TurboID ligase under the control of the constitutive promoter of the clock controlled gene 1 (ccg1) from Neurospora crassa. For the second control, we crossed the strain expressing the sci1-TurboID fusion gene (native sci1 promoter) with the Δpro11 (Bloemendal et al., 2012) deletion strain. The pro11 gene encodes a striatin homolog, which serves as the main scaffold of the SmSTRIPAK complex. Therefore, the second control setup enables the comparison of the proxiome of SCI1-TurboID in the presence or absence of the assembled SmSTRIPAK complex (Δpro11). We generated additional input controls of the crude protein extracts with aliquots taken before biotin affinity purification. Roux, K. J., Kim, D. in, Raida, M., & Burke, B. (2012). A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. The Journal of Cell Biology, 196(6), 801–810. Branon, T. C., Bosch, J. A., Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N., & Ting, A. Y. (2018). Efficient proximity labeling in living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880–887. Hollstein, L. S., Schmitt, K., Valerius, O., Stahlhut, G., & Pöggeler, S. (2022). Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora. Scientific Reports, 12(1), 17727. Bloemendal, S., Bernhards, Y., Bartho, K., Dettmann, A., Voigt, O., Teichert, I., Seiler, S., Wolters, D. A., Pöggeler, S., & Kück, U. (2012). A homologue of the human STRIPAK complex controls sexual development in fungi. Molecular Microbiology, 84(2), 310–323.
HostingRepository	PRIDE
AnnounceDate	2026-08-19
AnnouncementXML	Submission_2026-08-19_03:48:12.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Sordaria macrospora; NCBI TaxID: NEWT:5147;
ModificationList	biotinylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-08 03:15:39	ID requested
⏵ 1	2026-08-19 03:48:14	announced

10.1371/JOURNAL.PBIO.3003969;

submitter keyword: Sordaria macrospora, TurboID, STRIPAK, proximity labeling, BioID

Dr. Prof. Stefanie Pöggeler
contact affiliation	Georg-August-Universität Göttingen, Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms
contact email	spoegge@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069198
     1. Label: PRIDE project
     2. Name: Proximity labeling of the SmSTRIPAK complex in Sordaria macrospora using the SCI1 subunit as bait to identify its SmSTRIPAK-dependent microenvironment