PXD069169

PXD069169 is an original dataset announced via ProteomeXchange.

Title	Proteomic Insights into Extinction Memory Deficits in Stress-Susceptible Female Rats
Description	Stress exposure can disrupt fear extinction, which is a hallmark of some stress-related disorders. The underlying molecular mechanisms of impaired extinction, especially in females, remain poorly understood. In this study, we investigated proteomics changes in the infralimbic cortex, a region critical for fear suppression, in female rats exposed to single prolonged stress (SPS). One week after SPS exposure, adult female rats underwent auditory fear conditioning and extinction training and were classified as susceptible or resilient based on their extinction performance. Quantitative proteomics analysis using tandem mass tag labeling and bioinformatics revealed distinct proteins and pathways between groups. Susceptible rats exhibited distinct proteomic profiles in the infralimbic cortex. Several of the 53 differentially expressed proteins are associated with synaptic plasticity and memory, including neurogranin and microtubule-associated protein tau (MAPT). Pathway enrichment analysis identified alterations in synaptogenesis, clathrin-mediated endocytosis, calcium signaling, and chaperone-mediated autophagy. Functional validation using AAV-shRNA knockdown of neurogranin or MAPT in glutamatergic neurons of the infralimbic cortex improved extinction memory in SPS-exposed animals. Our findings suggest that dysregulated protein expression in the infralimbic cortex contributes to impaired extinction memory and traumatic stress susceptibility in female rats, offering insight into the neurobiological mechanisms underlying vulnerability to stress-related disorders.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:32:33.319.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069169
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Loyda Melendez
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-06 10:44:50	ID requested
⏵ 1	2026-02-08 16:32:34	announced

10.6019/PXD069169;

Irizarry-M, é, ndez N, Acosta-Pierantoni Y, Diaz-Vazquez A, Hern, á, ndez A, Col, ó, n M, Tosado-Rodr, í, guez EL, Cantres-Rosario YM, Roche-Lima A, Rodr, í, guez-De Jes, ú, s AE, Mel, é, ndez LM, Porter JT, Proteomic insights into extinction memory deficits in stress-susceptible female rats. Front Behav Neurosci, 19():1703714(2025) [pubmed]

10.3389/fnbeh.2025.1703714;

submitter keyword: tau protein, neurogranin, single prolonged stress,Quantitative proteomics, fear extinction, infralimbic cortex, stress susceptibility

Loyda M. Meléndez, phd
contact affiliation	Translational Proteomics Center, Research Capacity Core, Center for Collaborative Research in Health Disparities, Academic Affairs Deanship, University of Puerto Rico Medical Sciences, San Juan 00936, Puerto Rico Department of Microbiology and Medical Zoology, University of Puerto Rico Medical Sciences, San Juan, PR 00936, USA
contact email	lmelendezlab@gmail.com
lab head
Loyda Melendez
contact affiliation	University of Puerto Rico Medical Sciences Campus
contact email	lmelendezlab@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069169
     1. Label: PRIDE project
     2. Name: Proteomic Insights into Extinction Memory Deficits in Stress-Susceptible Female Rats