PXD069160

PXD069160 is an original dataset announced via ProteomeXchange.

Title	Microbial consortia mediating lignocellulose turnover and denitrification in eutrophic lake sediment enrichments
Description	Anthropogenic activities such as urbanization and agriculture can potentially pose a threat to neighboring freshwaters through nitrate and phosphorous contamination, which over time may lead to lake eutrophication. In such nitrogen-polluted environments, oxygen is depleted, and plants die and decompose. This enhances denitrifying microbes that respire under hypoxic/anoxic conditions by reducing nitrate instead of molecular oxygen and using plant remnants (lignocellulose) as carbon source. Microbial lignocellulose degradation has been well-studied for both aerobic- and anaerobic conditions; however, its degradation during denitrification remains largely unknown. Here we have applied a combination of gas kinetics and meta-omics techniques to enrich and analyze microbial communities from 10 eutrophic lakes to identify a set of core microbial metagenome-assembled genomes (MAGs) present in all the eutrophic lakes. We have further investigated their strategies and enzyme profiles for degrading lignocellulose under denitrifying conditions. We identified Pseudomonadota, Bacteroidota, Verrucomicrobiota, and Actinomycetota as the most abundant phyla and they were present in enrichments from all eutrophic lakes having a key role in denitrification and fermentation. Lignocellulose degradation was, however, dominated by species outside the core microbiome, i.e., there were differing key degraders between lakes, suggesting some level of lake-specialization. Among these we observed potential respiratory DNRA pathways, and they expressed a broad range of CAZymes targeting the various lignocellulose subfractions. Interestingly, many of the detected MAGs contained NO dismutases, enzymes postulated to convert NO to molecular oxygen and dinitrogen gas.
HostingRepository	PRIDE
AnnounceDate	2026-04-30
AnnouncementXML	Submission_2026-04-30_07:28:29.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Bacteria; NCBI TaxID: NEWT:2;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-06 06:39:32	ID requested
⏵ 1	2026-04-30 07:28:30	announced

Dataset with its publication pending

submitter keyword: Metaproteomics

Magnus Øverlie Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069160
     1. Label: PRIDE project
     2. Name: Microbial consortia mediating lignocellulose turnover and denitrification in eutrophic lake sediment enrichments