PXD069146

PXD069146 is an original dataset announced via ProteomeXchange.

Title	Inter-bacterial dynamics of biofilms: impact on the formation and structure of Campylobacter biofilms for the development of new innovative control strategies (J4-3088)
Description	Survival and persistence of pathogenic bacteria throughout the food production and supply chain, as well as the zoonoses they cause, represent a major public health concern. Patients with certain chronic diseases have an increased risk of severe infection with the novel coronavirus during the current pandemic, which highlights the urgent need for global control of pathogens, including Campylobacter bacteria, within the food chain. The pathogenic bacteria Campylobacter jejuni are the leading cause of human intestinal infections in developed countries. The incidence of campylobacteriosis is increasing, with the main sources being the consumption of inadequately heat-treated contaminated poultry and pork meat, as well as microbiologically contaminated drinking water. The ability to form biofilms is an important adaptive and defensive mechanism that enables bacterial survival on various surfaces and underlies their persistence and successful transmission, including of strains resistant to multiple antibiotics. A biofilm is a microbial community attached to different abiotic or biotic surfaces, such as materials in the food production chain, medical equipment, or environmental surfaces. On food and food-processing materials, food residues promote biofilm colonization, thereby supporting bacterial survival and creating a continuous source of contamination and host-to-host transmission. This indicates a remarkable and insufficiently explored biofilm dynamic that enables environmentally sensitive planktonic cells—lacking adaptive or survival mechanisms—to develop environmental resistance (the so-called “Campylobacter paradox”). This facilitates transmission from the environment to slaughterhouses, through the food production and distribution chain, or directly via environmental water to humans as hosts. Therefore, understanding the inter-bacterial dynamics of Campylobacter biofilms is crucial for developing new and innovative strategies to limit contamination and/or treat microbial infections. Campylobacter bacteria will serve as a model for developing novel approaches or strategies to reduce such infections through an in-depth understanding of biofilm formation, inter-bacterial communication dynamics within the biofilm, and mechanisms that do not promote antibiotic resistance. The project will first focus on the inter-bacterial dynamics between Campylobacter and probiotic as well as spoilage bacteria. We will identify the genes, key proteins, and fundamental mechanisms essential for bacterial surface attachment and inter-bacterial interactions within the biofilm. Furthermore, we will investigate the poorly defined architecture of Campylobacter biofilms and the structural components of polysaccharides, proteins, and extracellular DNA (eDNA) responsible for structural support and efficient intercellular communication within the microbial community. Since Campylobacter inter-bacterial communication is not well described, our research will target bacterial interactions through signaling molecules and genetic material exchange, enabling us to understand their roles in metabolism and social inter-bacterial interactions during biofilm formation. Understanding biofilm formation processes, together with the development of new strategies to prevent Campylobacter contamination of food, will reduce the transmission of resistance and contribute to safer food production. Additionally, we will define key mechanisms of inter-bacterial communication and biofilm formation, providing insight into bacterial motility, nutrient acquisition, and adhesion to various abiotic (e.g., polystyrene) and biotic (e.g., human cell lines) surfaces. The project will provide a comprehensive understanding of the relationship between biofilm formation, bacterial survival, and virulence, as well as the structure of the biofilm and the role of extracellular polymeric substances (EPS) as “interaction tools” for communication with other bacteria. The findings obtained will improve food handling, preparation, and storage practices, and address knowledge gaps regarding microbiological risks associated with biofilms. Consequently, this will contribute to reducing human and animal morbidity and the high costs linked to the consumption of contaminated food.
HostingRepository	PRIDE
AnnounceDate	2026-05-04
AnnouncementXML	Submission_2026-05-03_21:27:58.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Blaž Jug
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: NEWT:192222;
ModificationList	acetylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-05 19:13:57	ID requested
⏵ 1	2026-05-03 21:27:59	announced

10.1186/S13099-026-00827-3;

submitter keyword: LActococcus cremoris, biofilm,Campylobacter jejuni

Anja Klančnik
contact affiliation	Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
contact email	anja.klancnik@bf.uni-lj.si
lab head
Blaž Jug
contact affiliation	University of Ljubljana, Biotechnical Faculty, Department for Food Science and Technology, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
contact email	blaz.jug@bf.uni-lj.si
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD069146

PRIDE project URI

• PRIDE
  1. PXD069146
     1. Label: PRIDE project
     2. Name: Inter-bacterial dynamics of biofilms: impact on the formation and structure of Campylobacter biofilms for the development of new innovative control strategies (J4-3088)