PXD069104

PXD069104 is an original dataset announced via ProteomeXchange.

Title	Label-Free Quantitative Proteomics Data for NPC Permeability Barrier Dynamics
Description	This dataset contains label-free quantitative (LFQ) mass spectrometry analyses of proteins associated with affinity-purified isolated native Saccharomyces cerevisiae nuclear pore complexes (NPCs). The study compared wild type NPCs (+CP WT NPCs) with NPCs frozen at -80°C, or incubate in buffer at 4°C for the indicated days, which led to loss of the central plug (−CP WT NPCs). +CP WT NPCs were also compared to NPCs obtained from mutant strains where nearly half of the mass of FG repeats have been deleted (ΔFG), or increased by doubling the size of the FG region of Nsp1 (Nsp1FGx2). LFQ analyses indicated that the presence of the central plug is strongly associated with a persistent fraction of karyopherins (Kaps) and other factors residing within the NPC. In contrast, the quantities of other NPC components remained stable within the precision of the method for WT and mutant NPCs.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-06_08:21:02.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenzhu Zhang
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-02 18:04:25	ID requested
⏵ 1	2025-10-06 08:21:03	announced

Dataset with its publication pending

submitter keyword: NPC, central transporter,LFQ

Brian T. Chait
contact affiliation	The Rockefeller University
contact email	chait@rockefeller.edu
lab head
Wenzhu Zhang
contact affiliation	The Rockefeller Universoty
contact email	wzhang01@rockefeller.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD069104

PRIDE project URI

• PRIDE
  1. PXD069104
     1. Label: PRIDE project
     2. Name: Label-Free Quantitative Proteomics Data for NPC Permeability Barrier Dynamics