PXD069101 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Harnessing Mass Spectrometry-Based Proteomics for Continuous Directed Evolution |
| Description | Continuous directed evolution is a powerful Synthetic Biology tool to engineer proteins with desired functions in vivo. Mimicking natural evolution, it involves repeated cycles of high-frequency mutagenesis, selection, and replication within platform cells, where the function of the target gene is tightly linked to the host cell’s fitness. However, cells might escape the selection pressure due to the inherent flexibility of their metabolism, which allows for adaptation. Whole-proteome analysis as well as targeted proteomics offer valuable insights into global and specific cellular changes. They can identify modifications in the target protein and its interactors to help understand its evolution and network integration. Using the continuous evolution of the Arabidopsis methionine synthases AtMS1 and AtMS2 as an example we show how mass spectrometry-based proteomics was able to assess the abundance of target enzymes, identify flaws in population construction, measure methionine metabolic adaptation and allow informed decision making in the evolution campaign. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-05 |
| AnnouncementXML | Submission_2025-12-04_19:22:25.473.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD069101 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Katharina Belt |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion; TSQ Altis |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-02 17:02:30 | ID requested | |
| ⏵ 1 | 2025-12-04 19:22:25 | announced | |
Publication List
Keyword List
| submitter keyword: Spectrometry-based proteomics |
| continuous directed evolution |
| OrthoRep |
| Arabidopsis methionine synthase |
| enzyme longevity |
Contact List
| Harvey Millar |
|---|
| contact affiliation | UWA, School of Molecular Sciences |
| contact email | harvey.millar@uwa.edu.au |
| lab head | |
| Katharina Belt |
|---|
| contact affiliation | University of Western Australia |
| contact email | katharina.belt@uwa.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD069101 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069101
- Label: PRIDE project
- Name: Harnessing Mass Spectrometry-Based Proteomics for Continuous Directed Evolution
