PXD069089

PXD069089 is an original dataset announced via ProteomeXchange.

Title	Coordination of nuclear RNA processing by speckle-localized kinase TAOK2
Description	Nuclear speckles are membraneless organelles that act as active splicing hubs especially at sites of high transcription; emerging views of this dynamic subnuclear structure place it as a hub of RNA processing from transcription to export. To manage a complex microcosm of RNA metabolism, nuclear speckles also require phosphorylation and kinases to execute their functions. We identified a nuclear speckle-localized kinase, TAOK2, as able to mediate the splicing and export of a transcript at the nuclear speckle. To characterize its cellular regulatory capacity at the endogenous nuclear speckle, we used siRNA knockdown of both whole cell and nucleocytoplasmic fractions to characterize the complete endogenous effects of TAOK2. We found that TAOK2 knockdown can impact over 10% of the transcriptome. Cellular and biochemical phosphoproteomics reveals TAOK2 at the center of a nuclear speckle regulatory environment and identifies nuclear speckle scaffolding proteins SRRM1 and SRRM2 as potential direct phosphorylation targets mediating its large effects on the speckle. Indeed, knockdown of TAOK2 perturbs almost all serine/arginine (SR)-rich proteins while leaving heterogeneous ribonucleoproteins (HNRNPs) unperturbed. Altogether, we propose a structural maintenance role for phosphorylation by TAOK2 at SRRM1/2 that impacts SR protein-driven exon inclusion localized at the nuclear speckle.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:42:18.443.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	George M Burslem
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-02 07:42:19	ID requested
⏵ 1	2026-09-06 16:42:19	announced

10.1101/gad.353395.125;

Begg BE, Tracey MA, Gao S, Earnest S, Najar MA, Burslem GM, Cobb MH, Fontoura BMA, Lynch KW, Coordination of nuclear RNA processing by speckle-localized kinase TAOK2. Genes Dev, 40(15-16):1250-1266(2026) [pubmed]

submitter keyword: phosphoproteome, speckle-localized kinase TAOK2,nuclear RNA processing

George Burslem
contact affiliation	Dept of Biochemistry and Biophysics, Penn Medicine
contact email	george.burslem@pennmedicine.upenn.edu
lab head
George M Burslem
contact affiliation	University of Pennsylvania
contact email	george.burslem@pennmedicine.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD069089

PRIDE project URI

• PRIDE
  1. PXD069089
     1. Label: PRIDE project
     2. Name: Coordination of nuclear RNA processing by speckle-localized kinase TAOK2