PXD069084

PXD069084 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiles in cardiomyocytes exposed to ionizing radiation and resveratrol: potential radioprotective mechanisms of the different resveratrol pretreatment regimens
Description	Resveratrol, a plant-derived antioxidant, may protect against radiation by modulating cellular metabolism and response to stress. Here, we explored its effects on human cardiomyocytes exposed to ionizing radiation. Resveratrol (5 µM) was administered for 1, 7, and 30 days before a single 2 Gy dose irradiation, and then radiation toxicity and changes in the proteome were evaluated. Extended resveratrol treatment (7 or 30 days) induced more profound proteomic changes than one-day treatment and partially counteracted toxic effects, leading to increased cell survival, reduced cell death, and fewer cells arrested in the G1 phase. Though resveratrol administration itself had a greater impact on the proteome than radiation alone, we identified three subsets of proteins differently affected by radiation depending on the resveratrol context. The first subset represented the core cellular response to radiation, unaffected by resveratrol. The second, linked to DNA repair, cell cycle checkpoints, and apoptosis, was affected by radiation only in the absence of resveratrol preconditioning, indicating the compound's protective effect. The third subset involved in metabolism regulation was affected by radiation only after extended resveratrol preconditioning. In conclusion, our data documented that the radioprotective effects of resveratrol are linked to a proteomic reprogramming of cardiomyocytes following prolonged exposure.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_19:08:18.350.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069084
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Monika Pietrowska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-02 05:54:19	ID requested
⏵ 1	2025-11-09 19:08:19	announced

10.6019/PXD069084;

Gramatyka M, Gawin M, Kurczyk A, G, ą, dek A, Pietrowska M, Wid, ł, ak P, Proteomic Profiling of Cardiomyocytes Revealed Potential Radioprotective Effects of Different Resveratrol Pretreatment Regimens. Int J Mol Sci, 26(20):(2025) [pubmed]

10.3390/ijms262010223;

submitter keyword: radiation

resveratrol

radioprotection

proteomics

Monika Pietrowska
contact affiliation	Center for Translational Research and Molecular Biology of Cancer Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch 44-102 Gliwice, ul. Wybrzeże Armii Krajowej 15, Poland
contact email	monika.pietrowska@gliwice.nio.gov.pl
lab head
Monika Pietrowska
contact affiliation	Maria Sklodowska-Curie National Research Institute of Oncology
contact email	monika.pietrowska@io.gliwice.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069084

PRIDE project URI

• PRIDE
  1. PXD069084
     1. Label: PRIDE project
     2. Name: Proteomic profiles in cardiomyocytes exposed to ionizing radiation and resveratrol: potential radioprotective mechanisms of the different resveratrol pretreatment regimens