PXD069074

PXD069074 is an original dataset announced via ProteomeXchange.

Title	Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy
Description	Treatment of lung adenocarcinomas (LUAD) that exhibit activated epidermal growth factor receptor (EGFR) with EGFR tyrosine kinase inhibitors (TKI) has limited efficacy. Assessment of the impact of EGFR TKI on the LUAD surfaceome remodeling revealed potential therapeutic targets. We identified placental type alkaline phosphatase (ALPP) which has restricted expression in normal tissues, among upregulated surface proteins following EGFR TKI treatment of both TKI sensitive as well as resistant cells. Upregulation of ALPP with EGFR TKI resulted from activation of FoxO3a. The combination of EGFR TKI plus ALPP antibody conjugated with monomethylauristatin F enhanced tumor killing in osimertinib-sensitive and osimertinib-resistant LUAD models compared to either treatment alone. Our findings point to surfaceome remodeling induced with an established cancer therapy as a means for the development of more effective combination therapies.
HostingRepository	PRIDE
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2025-10-09_10:13:31.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yining Cai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-02 04:05:37	ID requested
⏵ 1	2025-10-09 10:13:31	announced

Dataset with its publication pending

submitter keyword: Placental type alkaline phosphatase, EGFR Tyrosine kinase inhibitors, Antibody drug conjugate, EGFR TKI resistance,Lung adenocarcinomas, Combinational therapy

Samir Hanash
contact affiliation	Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
contact email	shanash@mdanderson.org
lab head
Yining Cai
contact affiliation	Software Developer
contact email	ycai4@mdanderson.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD069074

PRIDE project URI

• PRIDE
  1. PXD069074
     1. Label: PRIDE project
     2. Name: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy