PXD069065

PXD069065 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of saliva, salivary stones and kidney stones reveals candidate biomarkers of pathological calcification
Description	Formation of salivary stones, a rare pathological condition, currently lacks non-surgical treatment options. The underlying causes remain unknown, although one hypothesis suggests bacterial infections may play a role. To investigate these theories and identify proteins whose altered levels might impact salivary gland homeostasis, leading to stone formation, optimal sample processing protocols were developed. The objective was to maximise protein extraction efficiency, evaluating sonication enhancement for this purpose. To establish a universal protocol applicable to various biological materials, different sample types—saliva, salivary stones, kidney stones, salivary glands, and Raoultella ornithinolytica—were processed and analysed. Since sonication increased the number of detected proteins, the subsequent step involved assessing the feasibility of quantitative analysis. Proteins identified in saliva and salivary stones were compared, revealing seventy-four overlapping proteins primarily associated with bacterial infections and extracellular regions, aligning with previous findings from salivary stone analysis alone. It suggests the potential for selecting high-confidence biomarkers responsible for stone formation, particularly by analysing saliva from healthy donors as a control group. Furthermore, 56 proteins were detected in both salivary stones and kidney stones, suggesting the potential to identify proteins crucial for stone formation across various diseases or pathological states.
HostingRepository	PRIDE
AnnounceDate	2026-03-11
AnnouncementXML	Submission_2026-03-11_07:11:33.296.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalia Musiał
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Raoultella ornithinolytica; NCBI TaxID: NEWT:54291;
ModificationList	monohydroxylated residue; formylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 22:53:19	ID requested
⏵ 1	2026-03-11 07:11:33	announced

Dataset with its publication pending

submitter keyword: Proteomics

Saliva

Salivary stones

Kidney stones

Biomarkers

Biocalcification

Sample preparation

Sonication

Paulina Czaplewska
contact affiliation	Laboratory of Mass Spectrometry Intercollegiate Faculty of Biotechnology UG-MUG Abrahama 58 80-307 Gdansk, Poland
contact email	paulina.czaplewska@ug.edu.pl
lab head
Natalia Musiał
contact affiliation	Intercollegiate Faculty of Biotechnology UG&MUG
contact email	natalia.musial@phdstud.ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD069065

PRIDE project URI

• PRIDE
  1. PXD069065
     1. Label: PRIDE project
     2. Name: Proteomic profiling of saliva, salivary stones and kidney stones reveals candidate biomarkers of pathological calcification