PXD069063

PXD069063 is an original dataset announced via ProteomeXchange.

Title	FAM134B clustering by liquid-like condensates facilitates RhoA sequestration and cell morphology
Description	The extensive cytoplasmic regions of ER-phagy receptors suggest integration of ER remodelling with cytoplasmic changes. Here, we present a cytoplasmic interactome of prominent receptors. Within this, regulatory interactors of FAM134B/C are identified by targeted CRISPR/Cas9 screening. We identify PRKAR1A as a direct activator of FAM134B/C, binding through an amphipathic helix within the otherwise disordered C-terminal region of FAM134B. Super-resolution, FRAP and CLEM imaging demonstrate that irregular, liquid-like condensates of cAMP-bound PRKAR1A form ER contacts, promoting clustering of FAM134B with LC3B. Proximity proteomics reveal that cAMP induces FAM134B interaction with cytoplasmic RhoA, co-degradation within condensate-associated lysosomes, and reduction in peripheral actomyosin contractility. Physiologically, co-ordination of ER and cytoplasmic remodelling is required for canonical cAMP-regulated cell morphologic changes, including determination of the matrix invasion modality of cancer cells. In summary, bidirectional cross-talk between the ER and the cytoplasm is facilitated by FAM134B/C and cytoplasmic liquid condensates.
HostingRepository	PRIDE
AnnounceDate	2026-06-17
AnnouncementXML	Submission_2026-06-17_11:48:49.575.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von Kriegsheim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 17:44:28	ID requested
⏵ 1	2026-06-17 11:48:50	announced

Dataset with its publication pending

submitter keyword: autophagy, bioID,human

ALex von Kriegsheim
contact affiliation	University of Edinburgh
contact email	avonkri@ed.ac.uk
lab head
Alex von Kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069063
     1. Label: PRIDE project
     2. Name: FAM134B clustering by liquid-like condensates facilitates RhoA sequestration and cell morphology