PXD069059

PXD069059 is an original dataset announced via ProteomeXchange.

Title	Proteomic and morphologic characterization of ovine macrophage differentiation and polarization – cell lysates
Description	Macrophages play crucial roles in inflammation and tissue homeostasis, exhibiting phenotypic and functional plasticity that enables them to initiate, sustain, or resolve inflammation. To support the evidence-based selection of biomedically relevant preclinical models, this study comprehensively characterized ovine macrophage differentiation and polarization by integrating morphological assessments with in-depth proteomic profiling of cellular lysates and secretomes. Monocytes isolated from peripheral blood were differentiated into macrophages using GM-CSF (GMØ) or M-CSF (MMØ), then polarized into M1 and M2 phenotypes. Mass spectrometry identified 4804 proteins in cell lysates and 901 in secretomes, including 42 CD antigens, enabling the establishment of CD marker profiles for monocytes and the distinct macrophage differentiation and polarization states. Proteomic analyses revealed significant upregulation of inflammatory markers in M1 and elevated tissue repair markers in M2 macrophages. Enrichment analysis confirmed activation of antimicrobial and matrix-degrading pathways in M1 macrophages, and regenerative, proteolysis-inhibiting functions in M2 macrophages. In conclusion, this study addresses the limitations of currently available immunological tools by providing an antibody-independent alternative for the classification of ovine macrophages, facilitating more precise phenotypic characterization and functional insight in ovine immunology research.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-12_03:31:23.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Ovis aries; NCBI TaxID: NEWT:9940;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 16:49:17	ID requested
⏵ 1	2026-01-12 03:31:24	announced

Elkhamary A, Gerner C, Bileck A, Gerner I, Jenner F, Proteomic and morphologic characterization of ovine macrophage differentiation and polarization. Sci Rep, 16(1):693(2025) [pubmed]

10.1038/s41598-025-30269-x;

submitter keyword: proteomics, macrophage differentiation, tissue repair,Inflammation, sheep

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD069059

PRIDE project URI

• PRIDE
  1. PXD069059
     1. Label: PRIDE project
     2. Name: Proteomic and morphologic characterization of ovine macrophage differentiation and polarization – cell lysates