PXD069031

PXD069031 is an original dataset announced via ProteomeXchange.

Title	The antiviral GTPase MxB is packaged into virions and binds via its N-terminal domain to alphaherpesvirus capsids
Description	The interferon-inducible myxovirus resistance proteins belong to the dynamin-like GTPases, with MxA being a restriction factor against several RNA viruses, while MxB restricts infections of lentiviruses and herpesviruses. Humans express MxA(1-662) with an N-terminal domain (NTD) of 43 residues, MxB(1-715) with an NTD of 91 residues, and a truncated MxB(26-715) with an NTD of 66 residues. Although the roles of the GTPase and stalk domains during infection are increasingly elucidated, the function of the different NTDs is not fully understood. Using cell lines stably expressing Mx proteins, we show in multi-step growth curves at low multiplicity of infection that MxB(1-715) and, less so, MxB(26-715) and MxA inhibited infection with herpes simplex virus (HSV-1), while MxB(1-715) and MxB(25-715) restricted pseudorabies virus (PrV) to a similar extent. Quantitative mass spectrometry and subviral fractionation experiments indicate that MxB(1-715), but not MxB(26-715) or MxA(1-662), enriched with the tegument of HSV-1 and PrV virions.. Moreover, we generated recombinant proteins with different NTDs and showed that MxB(1-91), MxB(1-35), and to a lesser extent MxB(26-91) bound to isolated HSV-1 capsids, while MxA(1-43) did not. Our findings indicate that the NTD of MxB is crucial to restrict HSV-1 and PrV infections, that newly assembled virions package MxB into the tegument around the capsid, and that the N-terminal and the C-terminal part of the MxB-NTD contribute to its binding to herpesviral capsids.
HostingRepository	PRIDE
AnnounceDate	2026-06-30
AnnouncementXML	Submission_2026-06-30_00:37:27.847.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boris Bogdanow
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 03:41:58	ID requested
⏵ 1	2026-06-30 00:37:28	announced

Dataset with its publication pending

submitter keyword: Herpes Simplex Virus, MxB, Virion

Fan Liu
contact affiliation	Leibniz-Institute for Molecular Pharmacology
contact email	fliu@fmp-berlin.de
lab head
Boris Bogdanow
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
contact email	bogdanow@fmp-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069031
     1. Label: PRIDE project
     2. Name: The antiviral GTPase MxB is packaged into virions and binds via its N-terminal domain to alphaherpesvirus capsids