PXD069030

PXD069030 is an original dataset announced via ProteomeXchange.

Title	A proteomics approach towards Ca2+ dependent and independent changes in response to oxidative stress in Arabidopsis thaliana
Description	Calcium (Ca2+) and reactive oxygen species (ROS) are key secondary messengers in plant stress signaling, yet their interplay in regulating proteome-wide responses remains poorly understood. We employed label-free quantitative (LFQ) proteomics to investigate Ca2+-dependent and independent proteome changes in Arabidopsis thaliana leaves upon oxidative stress induced by hydrogen peroxide (H2O2). To dissect the role of Ca2+ signaling, we inhibited H2O2-induced Ca2+ transients by pretreatment with LaCl3, a plasma membrane Ca2+ channel blocker. Throughoutall four treatment samples – control, H2O2 treated, LaCl3 treated, H2O2 and LaCl3 treated, we identified a total of 3724 and 3757 proteins after 10 and 30 min, respectively.
HostingRepository	PRIDE
AnnounceDate	2026-01-13
AnnouncementXML	Submission_2026-01-13_07:03:06.422.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annelotte van Dieren
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 03:26:17	ID requested
⏵ 1	2026-01-13 07:03:07	announced

10.1007/S00425-025-04891-Y;

submitter keyword: Arabidopsis thaliana, LC-MS analysis, calcium signaling, oxidative stress

Prof. Dr. Ute C. Vothknecht
contact affiliation	Institute for Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115 Bonn, Germany
contact email	vothknecht@uni-bonn.de
lab head
Annelotte van Dieren
contact affiliation	University of Bonn
contact email	annelotte.vandieren@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069030
     1. Label: PRIDE project
     2. Name: A proteomics approach towards Ca2+ dependent and independent changes in response to oxidative stress in Arabidopsis thaliana