PXD069025

PXD069025 is an original dataset announced via ProteomeXchange.

Title	A Bacillales-specific tubular scaffold essential for NADH dehydrogenase activity
Description	Respiratory type II NADH:quinone oxidoreductases (NDH-II) are typically monotopic flavoproteins that make direct contact with the membrane to access the quinone pool. Here, we show that in Bacillus subtilis, one NDH-II, termed Ndh, assembles with the helical membrane plugin (HMP) protein YjlC and forms supramolecular fibers. Genetic and biochemical analyses demonstrate that Ndh and YjlC proteins are essential for NADH oxidation. Cryo-EM analysis reveals that YjlC forms a tubular scaffold onto which multiple Ndh subunits are regularly docked via their C-terminal domain, repurposed from its classical role in direct membrane attachment. These fibers can extend up to ~1,000 Å, creating a continuous hydrophobic tunnel filled with lipids and quinones, thereby mimicking the membrane environment. Comparative genomics unveils that this partnership arose exclusively within Bacillales through the recruitment of an ancestral HMP originally associated with sulfide:quinone reductases. Together, our findings uncover a lineage-specific structural adaptation in which NDH-II enzymes depend on an HMP scaffold, expanding their functional diversity beyond the classical monotopic paradigm.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:10:48.288.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salomé Sauvage
SpeciesList	scientific name: Escherichia coli (strain K12); NCBI TaxID: NEWT:83333;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 01:17:49	ID requested
⏵ 1	2026-09-06 16:10:49	announced

Seduk F, Osman R, Garcia PS, Bizien-Jaglin L, Juyoux P, Kosta A, Sauvage S, Mat, é MJ, Pierrel F, Lebrun R, Schoehn G, Yamaryo-Bott, é Y, Bott, é CY, Nicolet Y, Cherrier MV, Walburger A, Magalon A, A Bacillales-specific tubular scaffold essential for NADH dehydrogenase activity. Nat Commun, 17(1):(2026) [pubmed]

10.1038/s41467-026-74385-2;

submitter keyword: NADH dehydrogenase

Bacillales

helical membrane plugin proteins

electron transfer chains, quinone-reacting oxidoreductase

Axel Magalon
contact affiliation	Laboratoire de Chimie Bacterienne, UMR7283 CNRS-AMU, 31 Chemin Joseph Aiguier, 13009 Marseille, France
contact email	magalon@imm.cnrs.fr
lab head
Salomé Sauvage
contact affiliation	Proteomics Facility of Institut de Microbiologie de la Méditerranée, CNRS, 31 Chemin Joseph Aiguier, 13009 Marseille
contact email	salome.sauvage@orange.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD069025

PRIDE project URI

• PRIDE
  1. PXD069025
     1. Label: PRIDE project
     2. Name: A Bacillales-specific tubular scaffold essential for NADH dehydrogenase activity