PXD069014 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MBD8 is required for LDL2-mediated transcriptional repression downstream of H3K9me2 in Arabidopsis |
| Description | Histone H3 lysine 9 methylation (H3K9me) is a conserved epigenetic mark for transcriptional gene repression. In Arabidopsis, H3K9 dimethylation (H3K9me2) promotes the removal of a transcription-associated mark, H3K4 monomethylation (H3K4me1), and this process within genes requires the histone demethylase LDL2. However, how LDL2 specifically functions in genes that accumulate H3K9me2 remains unknown. Here, we show that methyl-CpG-binding domain protein 8 (MBD8) binds to LDL2 and plays a role in H3K4me1 removal and transcriptional gene repression downstream of H3K9me2. MBD8 preferentially binds GC-poor DNA, regardless of its cytosine methylation status. Interestingly, LDL2 protein levels were significantly reduced in the mbd8 mutant background, suggesting that MBD8 stabilizes the LDL2 protein, facilitating H3K4me1 removal and the transcriptional repression of H3K9me-marked genes. The domain necessary for the MBD8-LDL2 interaction is conserved among plants. We propose that MBD proteins have diverse functions beyond their methylated DNA-binding capabilities. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-07_16:13:35.059.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shusei Mori |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-30 14:11:35 | ID requested | |
| ⏵ 1 | 2026-06-07 16:13:36 | announced | |
Publication List
| 10.1093/nar/gkag361; |
| Mori S, Osakabe A, Juliarni, Tanaka Y, Hirayama M, Inagaki S, Kakutani T, MBD8 is required for LDL2-mediated transcriptional repression downstream of H3K9me2 in Arabidopsis. Nucleic Acids Res, 54(9):(2026) [pubmed] |
Keyword List
| submitter keyword: Arabidopsis, LDL2, MBD8 |
Contact List
| Tetsuji Kakutani |
|---|
| contact affiliation | The University of Tokyo |
| contact email | tkak@bs.s.u-tokyo.ac.jp |
| lab head | |
| Shusei Mori |
|---|
| contact affiliation | University of Cambridge |
| contact email | sm3012@cam.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD069014 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069014
- Label: PRIDE project
- Name: MBD8 is required for LDL2-mediated transcriptional repression downstream of H3K9me2 in Arabidopsis
