PXD068960

PXD068960 is an original dataset announced via ProteomeXchange.

Title	Nuclear localization of glutaminyl-tRNA synthase QARS reveals its role in DNA damage repair in ovarian cancer
Description	Uncovering novel mechanisms controlling DNA damage response (DDR) is essential to better understand the molecular features of high-grade serous ovarian cancers (HGSOC), which remain a clinical challenge. Here we demonstrate that the glutaminyl-tRNA-synthetase QARS is a new key player in DDR. We show that QARS is regulated at translational level by the MAPK pathway in HGSOC and controls DDR through a translation-independent mechanism. Analyses combining RNA sequencing, immunohistochemistry, proteomic data and functional assays from HGSOC patient cohorts and relevant cellular models reveal that QARS promotes homologous recombination (HR)-mediated DNA repair in HGSOC, a function that requires its enzymatic activity. Interestingly, we uncover that QARS is located in the nucleus. Moreover, DNA damage increases QARS protein levels at the chromatin and its interaction with nuclear autoantigen SP100 and Dioxyuridine Triphosphatase DUT, involved in DNA break sensing and DNA integrity. Our data thus highlight a novel nuclear function and mechanism by which QARS enhances DDR in HGSOC.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_01:46:30.791.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Masson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-29 05:13:30	ID requested
⏵ 1	2026-05-20 01:46:31	announced

Dataset with its publication pending

submitter keyword: QARS, MAP kinases, DNA damage response,Ovarian cancer, Homologous recombination

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliation	Institut Curie - Centre de Recherche
contact email	vanessa.masson@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD068960

PRIDE project URI

• PRIDE
  1. PXD068960
     1. Label: PRIDE project
     2. Name: Nuclear localization of glutaminyl-tRNA synthase QARS reveals its role in DNA damage repair in ovarian cancer