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PXD068957

PXD068957 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Protein Complex Proteome of Phaeodactylum tricornutum organelles
DescriptionA native organelle-enriched fraction isolated from Phaeodactylum tricornutum was separated by two-dimensional Blue-native / SDS Polyacrylamid Gelelectrophoresis (2D BN / SDS PAGE). After vizualization of proteins on the gel with Coomassie, protein spots were cut, cysteine residues were carbamidomethylated and an in-gel digestion was carried out with trypsin over night. Peptides were extracted and used for HPLC-tandem MS using an online U3000 (Dionex) - Q Exactive Orbitrap MS system. Data were analyzed with MaxQuant (Version 2.6.8.0) and further processed in Excel (Microsoft Office). The final processed dataset was used to generate a reference map at www.gelmap.de (https://www.gelmap.de/5848).
HostingRepositoryMassIVE
AnnounceDate2026-01-22
AnnouncementXMLSubmission_2026-01-22_23:37:58.705.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJennifer Senkler
SpeciesList scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationListS-carboxamidomethyl-L-cysteine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-09-29 04:44:24ID requested
12026-01-22 23:37:59announced
Publication List
no publication
Keyword List
submitter keyword: Phaeodactylum, Polyacrylamid Gelelectrophoresis, Proteome, Protein Complexes, DatasetType:Proteomics
Contact List
Jennifer Senkler
contact affiliationLeibniz Universitaet Hannover
contact emailjsenkler@genetik.uni-hannover.de
lab head
Jennifer Senkler
contact affiliationLeibniz Universit?t Hannover
contact emailjsenkler@genetik.uni-hannover.de
dataset submitter
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MassIVE dataset URI
Dataset FTP location
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