PXD068927

PXD068927 is an original dataset announced via ProteomeXchange.

Title	Autoreactive T Cells Recognize Unique MBP Peptides Naturally Presented by EBV-Infected B Cells and Multiple Sclerosis Brain Tissue
Description	Epstein-Barr virus (EBV) is involved in causing and probably also perpetuating multiple sclerosis (MS). Among several mechanisms, how EBV may contribute, are transcriptome alterations including changes of antigen processing and preferential presentation of both viral and self-antigens. Here, we found that EBV reprograms the transcriptome and immunopeptidome presented on the MS-associated HLA-DR15 molecules of infected B cells. Identical myelin basic protein (MBP) peptides were found to be presented on both EBV-infected B cells and MS brain tissue but not primary B cells and thymic tissue. Peripheral memory and cerebrospinal fluid (CSF)-derived CD4+ T cells of HLA-DR15+ MS patients responded to MBP peptides, MBP(78-90) and/or MBP(83-90), and T cell clones raised with these peptides recognized all MBP peptides ending at amino acid MBP90 in MS brain tissues. Our study provides a new mechanistic link how the environmental and genetic risk factors, EBV infection and HLA-DR15 haplotype, may contribute jointly to MS.
HostingRepository	PRIDE
AnnounceDate	2026-06-19
AnnouncementXML	Submission_2026-06-19_04:19:23.906.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bernd Roschitzki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-28 22:49:07	ID requested
⏵ 1	2026-06-19 04:19:24	announced

10.1016/J.CELL.2025.12.046;

10.1016/j.cell.2025.12.046;

submitter keyword: MBP, EBV-infected B-cells,human, LC-MS/MS

Roland Martin
contact affiliation	Neuroimmunology and MS Research, Neurology Clinic, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland
contact email	roland.martin@uzh.ch
lab head
Bernd Roschitzki
contact affiliation	University of Zurich
contact email	bernd.roschitzki@fgcz.uzh.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068927
     1. Label: PRIDE project
     2. Name: Autoreactive T Cells Recognize Unique MBP Peptides Naturally Presented by EBV-Infected B Cells and Multiple Sclerosis Brain Tissue