PXD068909

PXD068909 is an original dataset announced via ProteomeXchange.

Title	Integrated tissue and serum proteomics on magnet-guided photothermal therapy using Au/Fe/Au trilayer nanodiscs
Description	Nanodiscs are emerging as promising nanomaterials for localized therapies as they enable magnetic targeting and near-infrared (NIR) laser–driven photothermal stimulation. In this study, we performed comprehensive proteomic analyses of both tissue and serum from mouse models to elucidate the effects of photothermal therapy using Au/Fe/Au trilayer nanodiscs (AuFeAuNDs) on both local and systemic protein regulation. Protein expression in tissue and serum was analyzed under six experimental exposure conditions: control, control plus laser irradiation, Au nanodiscs, AuFeAuNDs without magnetic induction, AuFeAuNDs with magnetic induction, and AuFeAuNDs with magnetic induction plus laser irradiation. Tissue proteomes showed enrichment of proteins involved in histone modification and transcriptional regulation, while serum proteomes revealed alterations in immune responses and coagulation pathways, with the combined AuFeAuNDs, magnet, and laser treatment promoting fibrinolysis. Fifty-two shared proteins across tissues and serum were implicated in ferroptosis, coagulation, and defense responses. Overall, these findings provide mechanistic insights into the biological impact and biosafety of nanodisc-mediated photothermal therapy, as well as highlight potential biomarkers for monitoring therapeutic responses.
HostingRepository	PRIDE
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_01:55:04.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Young Lee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-28 11:20:48	ID requested
⏵ 1	2025-10-15 01:55:05	announced

Dataset with its publication pending

submitter keyword: Photothermal therapy, Serum,Proteomics, Tissue, Nanodisc

Jin Gyeong Son
contact affiliation	Nanobio Measurement Group, Division of Biomedical Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea
contact email	yeskyoung@kriss.re.kr
lab head
Sun Young Lee
contact affiliation	Korea Research Institute of Standards and Science
contact email	sylee12@kriss.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068909
     1. Label: PRIDE project
     2. Name: Integrated tissue and serum proteomics on magnet-guided photothermal therapy using Au/Fe/Au trilayer nanodiscs