PXD068883

PXD068883 is an original dataset announced via ProteomeXchange.

Title	Proteomic and secretomic response of an African Armillaria species to iron.
Description	Armillaria species have attracted considerable research interest because they are widely distributed, mostly plant-pathogenic, and exhibit unique characteristics. Abiotic factors influence intra- and inter-species variations in pathogenicity and/or virulence of these fungi. However, the mechanisms involved in causing these variations are not well understood. Iron is an indispensable element in several molecular and biological processes. Yet, excessive abundance of iron can be toxic to organisms due to Fenton-like reactions. This study aimed to gain insights into the type and extent of iron-responsive proteomic and secretomic changes in Armillaria sp. strain CMW4456 cultured in liquid media supplemented with iron using a multi-omics approach. Significant iron-dependent alterations of proteins involved in metabolism and growth were observed in the proteomes and secretomes. Iron supplementation at 100µM did not elicit an oxidative stress response by the fungus. Our analyses revealed three putative siderophore biosynthetic gene clusters (BGCs) in the genome and expression of proteins encoded by some BGC genes in the proteome. This knowledge contributes to a better understanding of the mechanisms employed by an Armillaria sp. in response to iron, gives insights into possible modes for inhibiting or attenuating pathogenicity and/or virulence of Armillaria spp., and can be valorised for more biotechnological applications.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-02_03:28:02.303.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Deborah L. Narh
SpeciesList	scientific name: Basidiomycota; NCBI TaxID: NCBITaxon:5204; scientific name: Armillaria sp. CMW 4456; NCBI TaxID: NEWT:366497; scientific name: Fungi; NCBI TaxID: NCBITaxon:4751;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-26 16:05:39	ID requested
⏵ 1	2026-03-02 03:28:03	announced

10.1021/acs.jproteome.5c00979;

Narh DL, Wingfield BD, Coetzee MPA, Species to Iron. J Proteome Res, 25(3):1611-1625(2026) [pubmed]

submitter keyword: None

Brenda D.
contact affiliation	Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI),Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa
contact email	brenda.wingfield@fabi.up.ac.za
lab head
Deborah L. Narh
contact affiliation	Stanford University
contact email	dlnarh@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD068883

PRIDE project URI

• PRIDE
  1. PXD068883
     1. Label: PRIDE project
     2. Name: Proteomic and secretomic response of an African Armillaria species to iron.