PXD068874 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Neuroinflammatory Stress Preferentially Impacts Synaptic MAPK Signaling and Mitochondria in Excitatory Neurons |
| Description | In this study, we have applied cell type-specific in vivo biotinylation of proteins (CIBOP) to label Camk2a excitatory neuronal proteomes, and imposed differential centrifugation to prepare synaptosome-enriched fractions, followed by proteomic analysis of biotinylated neuronal proteins. This approach allowed us to obtain the native-state proteome of crude synaptosomes containing presynaptic (mitochondria and synaptic vesicles) and postsynaptic compartments of excitatory neurons from mouse brain, while directly contrasting them with the whole neuronal proteome. Next, we used the systemic LPS-induced neuroinflammation model to activate microglia in an AD-relevant manner, and examined how the synaptic compartment of excitatory neurons responds to neuroinflammatory stress, in contrast with the somatodendritic compartment. We observed that the effects of neuroinflammation on the synaptic compartment were indeed distinct from the somatodendritic compartment, suggestive of compartment-specific effects of neuroinflammation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-13 |
| AnnouncementXML | Submission_2026-04-12_16:58:29.844.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | UPASNA SRIVASTAVA |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-26 12:21:04 | ID requested | |
| ⏵ 1 | 2026-04-12 16:58:31 | announced | |
Publication List
| 10.1186/s44477-026-00024-1; |
| Espinosa-Garcia C, Srivastava U, Kumar P, Kour D, Malepati S, Tobin BR, Xiao H, Sunna S, Bowen CA, Cheng L, Bagchi P, Duong DM, Whitworth TJ, Liu X, Seyfried NT, Wood LB, Faundez V, Rangaraju S, Neuroinflammatory stress preferentially impacts synaptic MAPK signaling and mitochondria in excitatory neurons. Mol Neurodegener Adv, 2(1):17(2026) [pubmed] |
Keyword List
| submitter keyword: Mitochondria, Excitatory neuron, Neuroinflammation, Synapse vulnerability, Alzheimer's disease, Cognitive resilience, MAPK signaling,Native-state proteomics, Synaptosome |
Contact List
| UPASNA SRIVASTAVA |
|---|
| contact affiliation | Yale School of Medicine, Department of Neurolgy, Yale University |
| contact email | upasna.srivastava@yale.edu |
| lab head | |
| UPASNA SRIVASTAVA |
|---|
| contact affiliation | YALE UNIVERSITY |
| contact email | upasna.srivastava@yale.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068874
- Label: PRIDE project
- Name: Neuroinflammatory Stress Preferentially Impacts Synaptic MAPK Signaling and Mitochondria in Excitatory Neurons
