PXD068866

PXD068866 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Priming-Dependent Modulation of Secretome in Placenta- and Adipose-Derived Mesenchymal Stromal/Stem cells: Toward Cell-Free Immunoregenerative Therapies for Osteoarticular Disorders
Description	Age-related bone degeneration is driven by structural deterioration, chronic inflammation, and impaired regenerative capacity, leading to an increased incidence of osteoarticular disorders. Mesenchymal stromal/stem cells (MSCs) have emerged as promising therapeutic candidates due to their immunomodulatory and regenerative properties, mediated largely through their secretome and extracellular vesicles (EVs). However, the therapeutic efficacy of MSC-derived products depends on both the cell source and the conditioning stimuli to which MSCs are exposed. In this study, we performed a comparative proteomic and exosomal microRNA (miRNA) profiling of secretomes derived from placenta-derived MSCs (PSCs) and adipose-derived MSCs (ASCs), and assessed the impact of priming strategies, including hypoxia, interferon-gamma (IFNγ), and interleukin-1 beta (IL1β), on paracrine properties of these cells. Proteomic analysis identified over 7,000 proteins, with PSC secretome enriched in pathways related to osteogenesis, chondrogenesis, extracellular matrix organization, angiogenesis, and immune regulation, whereas ASC secretome displayed limited enrichment in these processes. Functional scoring highlighted IL1β priming as the most effective strategy to enhance osteochondral and immunomodulatory protein signatures in PSCs. Differently, IFNγ priming selectively expanded the repertoire of exosomal miRNAs, with enrichment in signaling networks including Wnt/β-catenin, TGF-β, NF-κB, and T cell receptor pathways, underscoring its role in fine-tuning immune and regenerative functions. Together, these findings reveal that PSCs secrete a broader and more functionally relevant spectrum of bioactive molecules for osteoarticular applications compared with ASCs. Moreover, distinct priming strategies can differentially modulate their paracrine outputs. IL1β primarily enhances protein-driven regenerative and immunomodulatory activity, whereas IFNγ promotes a functionally enriched exosomal miRNA cargo. These results support PSC-derived secretomes as versatile candidates for next-generation, cell-free therapies in osteoarticular disease.
HostingRepository	PRIDE
AnnounceDate	2026-06-16
AnnouncementXML	Submission_2026-06-15_23:40:59.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Margot Lo Pinto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-26 08:32:02	ID requested
⏵ 1	2026-06-15 23:41:00	announced

Publication List

Ligotti ME, Scalia E, Scilabra SD, Pinto ML, Gallo A, Vazzana R, Cuscino N, Re OL, Pipia C, Amico G, Lopa S, Colombini A, Ippolito V, Maurizi A, Rucci N, Agnese V, Conaldi PG, Moretti M, Miceli V, Bulati M, Priming-Dependent Modulation of Secretome in Placenta- and Adipose-Derived Mesenchymal Stromal/Stem Cells: Toward Cell-Free Immunoregenerative Therapies for Osteochondral and Degenerative Joint Diseases. Stem Cell Rev Rep, 22(5):2453-2473(2026) [pubmed]
10.1007/s12015-026-11122-8;

Ligotti ME, Scalia E, Scilabra SD, Pinto ML, Gallo A, Vazzana R, Cuscino N, Re OL, Pipia C, Amico G, Lopa S, Colombini A, Ippolito V, Maurizi A, Rucci N, Agnese V, Conaldi PG, Moretti M, Miceli V, Bulati M, Priming-Dependent Modulation of Secretome in Placenta- and Adipose-Derived Mesenchymal Stromal/Stem Cells: Toward Cell-Free Immunoregenerative Therapies for Osteochondral and Degenerative Joint Diseases. Stem Cell Rev Rep, 22(5):2453-2473(2026) [pubmed]

10.1007/s12015-026-11122-8;

Keyword List

submitter keyword: Placenta-derived MSCs, Exosomal microRNA,Mesenchymal stromal cells, Cytokine priming, Adipose-derived MSCs, Secretome, Proteomics, Extracellular vesicles, Osteogenesis, Chondrogenesis

submitter keyword: Placenta-derived MSCs, Exosomal microRNA,Mesenchymal stromal cells, Cytokine priming, Adipose-derived MSCs, Secretome, Proteomics, Extracellular vesicles, Osteogenesis, Chondrogenesis

Contact List

Simone Dario Scilabra
contact affiliation	Department of Laboratory Medicine and Advanced Biotechnologies, Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione (IRCCS - ISMETT) Via Ernesto Tricomi, 5 - 90127 Palermo
contact email	sdscilabra@fondazionerimed.com
lab head
Margot Lo Pinto
contact affiliation	Fondazione Ri.MED
contact email	mlopinto@fondazionerimed.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068866

PRIDE project URI

Repository Record List

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