PXD068848

PXD068848 is an original dataset announced via ProteomeXchange.

Title	Human Calcitonin and Amylin receptor HDX-MS
Description	Receptor activity-modifying proteins (RAMPs) regulate the pharmacology, trafficking, and signaling of class B1 G protein-coupled receptors (GPCRs). The calcitonin receptor (CTR) interacts with each of the three RAMPs to form amylin receptors (AMYRs), which display distinct ligand preferences and signaling outputs. While cryo-electron microscopy has described AMYR structures in active conformations, less is known about how RAMPs influence CTR dynamics in the absence of ligand. In this study, we applied hydrogen–deuterium exchange mass spectrometry (HDX-MS) to examine conformational dynamics of the CTR alone and in complex with RAMP1, RAMP2, or RAMP3 in their apo states. The data show that RAMPs alter the flexibility of several CTR regions, including extracellular loops, transmembrane helices, and intracellular segments associated with G protein binding. In addition, each RAMP subtype displays characteristic dynamic features within its transmembrane and C-terminal domains. These HDX-MS datasets provide mechanistic insight into how RAMPs modulate CTR structure and offer a resource that links static structural models with functional pharmacology.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_18:26:57.337.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tracy Josephs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-25 17:15:39	ID requested
⏵ 1	2026-06-07 18:26:58	announced

Fairweather CJ, Zhang X, Fernando CD, Garama DJ, Sexton PM, Wootten D, Josephs TM, Conformational Dynamics of Amylin Receptors Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry. J Am Chem Soc, 148(16):16702-16712(2026) [pubmed]

10.1021/jacs.5c20644;

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: calcitonin recepto,HDX-MS, recepror activity modifying protein (RAMP), GPCR, amylin receptor

Tracy Maree Josephs
contact affiliation	Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Parkville, Victoria, Australia
contact email	tracy.josephs@monash.edu
lab head
Tracy Josephs
contact affiliation	Monash University
contact email	tracy.josephs@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068848

PRIDE project URI

• PRIDE
  1. PXD068848
     1. Label: PRIDE project
     2. Name: Human Calcitonin and Amylin receptor HDX-MS