PXD068832

PXD068832 is an original dataset announced via ProteomeXchange.

Title	A Modular Chromosomal Passenger Complex Rewires Chromosome Segregation in Plasmodium berghei
Description	Faithful chromosome segregation relies on precise kinetochore-microtubule interactions and checkpoint surveillance, yet the molecular basis of these processes varies widely across eukaryotes and is only beginning to be defined in apicomplexan parasites. In the malaria parasite Plasmodium berghei, chromosome segregation is especially critical during transmission from host to mosquito: rapid mitoses generate male gametes, and subsequent meiosis in the zygote seeds the next generation of infection. Here, we identify Aurora-related kinase 1 (ARK1) as a central regulator of chromosome segregation in both mitotic and meiotic contexts. ARK1 localises to spindle poles, spindles, and kinetochores, and its depletion results in short and multipolar spindles, kinetochore misalignment, and failed chromosome partitioning. ARK1 forms a minimal Chromosomal Passenger Complex (CPC) with INCENP1 during male gametogenesis, but a more elaborate CPC with INCENP2, kinetochores, centromeric histones, and spindle assembly checkpoint proteins during meiosis. This stage-specific modularity allows Plasmodium to prioritise male gamete formation whilst safeguarding faithful chromosome inheritance during zygote development, ensuring parasite transmission to the mosquito. Our findings demonstrate that conserved CPC principles are rewired in Plasmodium, highlighting both the plasticity of eukaryotic checkpoint control and a potential vulnerability for blocking malaria transmission.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-06-01_04:26:46.512.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lorenzo Brusini
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: NEWT:5823;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-25 09:42:50	ID requested
⏵ 1	2026-06-01 04:26:47	announced

Dataset with its publication pending

submitter keyword: malaria, evolutionary cell biology, Apicomplexa, kinetochore,Aurora kinase, chromosomal passenger complex, Plasmodium

Lorenzo Brusini
contact affiliation	Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Switzerland
contact email	lorenzo.brusini@unige.ch
lab head
Lorenzo Brusini
contact affiliation	Université de Genève
contact email	lorenzo.brusini@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068832

PRIDE project URI

• PRIDE
  1. PXD068832
     1. Label: PRIDE project
     2. Name: A Modular Chromosomal Passenger Complex Rewires Chromosome Segregation in Plasmodium berghei