PXD068827 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows |
| Description | This study presents a comparative analysis of three LysC endopeptidases homologues from Achromobacter lyticus, Pseudomonas aeruginosa, and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture (PAC) workflow with HeLa cell lysate, we assessed the enzymes' cleavage specificity, digestion efficiency, and performance across various experimental conditions. Results showed that while all three LysC homologues exhibited high cleavage specificity at lysine residues, A. lyticus LysC outperformed the two others with its superior peptide identification, digestion efficiency, and protein coverage, especially at shorter digestion times. Combination of A. lyticus LysC and Trypsin demonstrated the importance of employing LysC for significantly minimizing missed cleavage rates in tryptic digests. This study underscores A. lyticus LysC's potential as an optimal choice for enhancing mass spectrometry-based proteomics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-23 |
| AnnouncementXML | Submission_2026-02-22_18:44:53.855.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leander Van der Hoeven |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-25 04:36:40 | ID requested | |
| ⏵ 1 | 2026-02-22 18:44:54 | announced | |
Publication List
| van der Hoeven LR, Lechner M, Hernandez-Rollan C, Batth TS, Olsen JV, Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows. J Proteome Res, 25(2):1176-1183(2026) [pubmed] |
| 10.1021/acs.jproteome.5c00872; |
Keyword List
| submitter keyword: Endoproteinase Lys-C, Proteomics workflow, Method development, Label-Free Quantitation, Bottom-up proteomics, proteolytic enzymes, LC-MS/MS |
Contact List
| Jesper Velgaard Olsen |
|---|
| contact affiliation | University of Copenhagen Faculty of Health and Medical Sciences Department of Cellular and Molecular Medicine Novo Nordisk Foundation Center for Protein Research (CPR) Proteomics Program Blegdamsvej 3B, DK-2200 Copenhagen N Denmark |
| contact email | Jesper.olsen@cpr.ku.dk |
| lab head | |
| Leander Van der Hoeven |
|---|
| contact affiliation | NNF - CPR |
| contact email | leander.van.der.hoeven@cpr.ku.dk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068827 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068827
- Label: PRIDE project
- Name: Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows
