PXD068826

PXD068826 is an original dataset announced via ProteomeXchange.

Title	Identification of interacting partners of CNPY1 in the mouse vomeronasal organ.
Description	Distinct families of V1R or V2R type GPCRs are expressed in the mouse vomeronasal organ within spatially segregated vomeronasal sensory neurons (VSNs) that are also identified by the expression of cognate G-protein subunits, Gαi2 or Gαo. We identify mouse Cnpy1 as a novel VNO specific ER chaperone, with expression restricted to Gαo VSNs. Absence of Cnpy1 leads to reduced neuronal activation, behavioral defects and a substantial loss of neurons by apoptosis during postnatal development. To identify interacting partners of Cnpy1, we immunoprecipitated Cnpy1 from lysate of C57BL/6 (B6) VNOs using anti-Cnpy1 antibody (IPCnpy1:B6). As controls, we performed IP from Cnpy1-/- VNO lysate (IPCnpy1:KO) as well as an IP using normal IgG from B6 VNO lysate (IPIgG:B6). The protein eluates from all IP samples were fragmented to peptides using trypsin and labeled with TMT. We pooled TMT labeled samples and performed fractionation followed by liquid chromatography-quantitative mass spectrometry to identify the co-immunoprecipitating interacting partners of Cnpy1. Hits from IPCnpy1:KO and IPIgG:B6 serve as negative controls to identify non-specific interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2026-03-13
AnnouncementXML	Submission_2026-03-13_09:03:26.835.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-25 04:24:34	ID requested
⏵ 1	2026-03-13 09:03:27	announced

Dataset with its publication pending

submitter keyword: mouse, Cnpy1, vomeronasal organ

Adish Dani
contact affiliation	Tata Institute of Fundamental Research, Hyderabad, India.
contact email	adish.d@gmail.com
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD068826

PRIDE project URI

• PRIDE
  1. PXD068826
     1. Label: PRIDE project
     2. Name: Identification of interacting partners of CNPY1 in the mouse vomeronasal organ.