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PXD068824

PXD068824 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein-protein interactions of PID-3 in C. briggsae
DescriptionA mass spectrometry-based label-free proteomics analysis was performed to study the protein binders of PID-3 in C. briggsae. Anti-PID-3 and IgG control pull-downs were performed on C. briggsae. Proteins were digested in-gel by trypsin. Peptides were measured on a Q Exactive Plus Orbitrap mass spectrometer. Raw data files were processed by MaxQuant software package (version 2.1.3.0) using its integrated Andromeda search engine. Spectral data were searched against a target-decoy database consisting of the forward and reverse sequences of the UniProt C. briggsae (release 2023_02; 21,756 entries) and E. coli (release 2023_01; 5,064 entries) reference proteomes and a list of common contaminants. False discovery rate (FDR) was set to 1% at both peptide and protein levels. MaxLFQ quantification algorithm was employed.
HostingRepositoryMassIVE
AnnounceDate2025-11-13
AnnouncementXMLSubmission_2025-11-13_07:17:26.270.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJ Chen
SpeciesList scientific name: Caenorhabditis briggsae; NCBI TaxID: 6238;
ModificationListiodoacetamide - site C
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-09-25 03:09:47ID requested
12025-11-13 07:17:26announced
Publication List
no publication
Keyword List
submitter keyword: Protein-protein interactions, C. briggsae, PID-3, DatasetType:Proteomics
Contact List
Rene Ketting
contact affiliationInstitute of Molecular Biology, Mainz
contact emailR.Ketting@imb-mainz.de
lab head
J Chen
contact affiliationInstitute of Molecular Biology
contact emailj.chen@imb-mainz.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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