PXD068821 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex |
| Description | Mitosis in Plasmodium spp., the causative agent of malaria, is fundamentally different from model eukaryotes, proceeding via a bipartite microtubule organising centre (MTOC) and lacking canonical regulators such as Polo and Bub1 kinases. During schizogony, asynchronous nuclear replication produces a multinucleate schizont, while rapid male gametogony generates an octaploid nucleus before gamete formation. Here, we identify Aurora-related kinase 1 (ARK1) as a key component of inner MTOC and spindle formation, controlling kinetochore dynamics and driving mitotic progression. Conditional ARK1 depletion disrupts spindle biogenesis, kinetochore segregation, karyokinesis and cytokinesis in both stages, and affects parasite transmission. Interactome analysis reveals ARK1 as the catalytic core of a non- canonical chromosomal passenger complex (CPC) containing two divergent inner centromere proteins (INCENPs) but lacking Survivin and Borealin. Comparative genomics indicates this CPC architecture arose early in Apicomplexa, replacing canonical centromere-targeting modules. These findings uncover a distinct mitotic machinery in Plasmodium and identify the ARK1–INCENP interface as a potential multistage target for malaria therapeutic intervention. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-13 |
| AnnouncementXML | Submission_2026-01-13_07:53:30.212.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD068821 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Andrew Bottrill |
| SpeciesList | scientific name: Plasmodium berghei ANKA; NCBI TaxID: NEWT:5823; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro; Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-25 01:41:02 | ID requested | |
| ⏵ 1 | 2026-01-13 07:53:30 | announced | |
Publication List
Keyword List
| submitter keyword: male gametogony,Plasmodium, Aurora kinase, cytokinesis, Warwick_RTP, spindle, chromosomal passenger complex (CPC), INCENP, karyokinesis, MTOC, transmission |
Contact List
| Professor Rita Tewari |
|---|
| contact affiliation | Faculty of Medicine and Health Sciences School of Life Sciences University of Nottingham NG7 2UH United Kingdom |
| contact email | rita.tewari@nottingham.ac.uk |
| lab head | |
| Andrew Bottrill |
|---|
| contact affiliation | University of Warwick |
| contact email | a.r.bottrill@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD068821 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068821
- Label: PRIDE project
- Name: Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex
