PXD068804

PXD068804 is an original dataset announced via ProteomeXchange.

Title	Proteomics in retinal degeneration in a mouse model bearing a pde6β mutation
Description	Retinitis pigmentosa (RP) is a progressive and degenerative retinal disease that results in severe loss of vision. Models of RP have been extensively studied for pathogenetic mechanisms and treatments. Yet there is little information about the alterations of RP associated proteins and pathways in pde6b mutated mouse model. To explore the roles of RP causing proteins, we performed a label free quantitative mass spectrometry based proteomic analysis in rd10 mouse retinas at 5 weeks and 8 weeks. A total of 3737 proteins were identified at the degenerative time points in rd10 mice. A 2-fold change in 222 proteins was observed at 5 weeks, and a 2-fold change in 289 proteins was detected at 8 weeks. Based on differentially expressed proteins (DEPs) enriched in GO and KEGG pathway analysis, visual perception and phototransduction were severely affected. The pathways for cilium assembly and protein localization were found to be downregulated. Moreover, heatmaps and protein-protein interaction network analysis of 25 DEPs (fold change>2, p<0.05) causing autosomal recessive/dominant retinitis pigmentosa demonstrated protein abundance and the mutually potential interactions. A total of thirteen proteins interact directly with the Pde6b protein. The 25 DEPs responsible for RP were involved with phototransduction, visual perception, response to stimulus, protein localization and cilium assembly pathways. Their expressions were validated by qPCR and western-blot. This study provided an analysis and verification of DEPs causing RP in pde6b mutated RP mouse model. Our work shed light on the molecular mechanisms underlying RP, which pointed direction for RP therapies in future.
HostingRepository	iProX
AnnounceDate	2025-09-24
AnnouncementXML	Submission_2025-09-24_19:16:11.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiuxiu Jin
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	QTRAP 6500+

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 19:15:53	ID requested
⏵ 1	2025-09-24 19:16:12	announced

Dataset with its publication pending

submitter keyword: retinitis pigmentosa, proteomics, pde6β

Bo Lei
contact affiliation	Henan Provincial People's Hospital
contact email	bolei99@126.com
lab head
Xiuxiu Jin
contact affiliation	Henan Provincial People's Hospital
contact email	jindaxiu@163.com
dataset submitter

iProX dataset URI