PXD068799

PXD068799 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry proteomics and glycoproteomics data from human body fluids
Description	The study of proteins and N-glycosylation in human body fluids (HBFs) is crucial for understanding both physiological and pathological states, offering valuable insights for disease diagnostics and monitoring. In this study, we present a comprehensive proteomics and glycoproteomics dataset featuring 15 types of HBFs (plasma (P), urine (U), cerebrospinal fluid (CSF), pleural fluid (PF), ascitic fluid (AF), synovial fluid (SF), pericardial fluid (PCF), peritoneal dialysis effluent (PDE), bile (B), gastric juice (GJ), seminal plasma (SP), saliva (SA), pancreatic juice (PJ), sweat (SW), and tear (T)). We also provide detailed protocols for protein processing specific to each type of HBF. The re sulting peptides and enriched intact N-glycopeptides were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data independent acquisition (DIA) and combined electron transfer/higher-energy collisional dissociation and stepped collision energy/higher-energy collisional dissociation (EThcD-sceHCD) mode, respectively. The resulting spectra and searched files have been uploaded to online repository iProX (www.iprox.cn) and made easily accessible to facilitate further research into HBF proteomics and glycoproteomics.
HostingRepository	iProX
AnnounceDate	2025-09-25
AnnouncementXML	Submission_2025-09-24_18:29:24.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	deaminated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 18:29:08	ID requested
⏵ 1	2025-09-24 18:29:24	announced

Dataset with its publication pending

submitter keyword: Human body fluid, Proteomics, Glycoproteomics, N-glycosylation, Intact N-glycopeptide.

Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
lab head
Yang Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
dataset submitter

iProX dataset URI