PXD068796

PXD068796 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 Nsp3 interactome in Caco-2 cells during authentic infection
Description	The nonstructural protein 3 (Nsp3) of SARS-CoV-2 is a large, multi-domain protein that localizes to double-membrane vesicles (DMVs), the physical site where SARS-CoV-2 (and other coronaviruses) replicate inside mammalian cells. One of the domains of Nsp3 is PLpro (papain-like protease), which harbors enzymatic activities (i.e., protease, deubiquitinase, and deISGylase). To identify human cellular proteins that bind Nsp3, we performed LC-MS/MS analysis of affinity-purified Nsp3 from SARS‑CoV‑2–infected Caco-2 (human intestinal epithelial) cells. This dataset identified numerous Nsp3-interacting proteins that play key roles in various host cellular processes, including metabolism and innate immunity.
HostingRepository	PRIDE
AnnounceDate	2026-05-06
AnnouncementXML	Submission_2026-05-05_16:46:23.697.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junji Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 17:17:15	ID requested
⏵ 1	2026-05-05 16:46:24	announced

Dataset with its publication pending

submitter keyword: Nsp3,SARS-CoV-2, coronavirus, interactome, Caco-2, LC-MS/MS

Michaela Gack
contact affiliation	Florida Research and Innovation Center, Cleveland Clinic, Port Saint Lucie, FL 34987, USA
contact email	GACKM@ccf.org
lab head
Junji Zhu
contact affiliation	Florida Research and innovation center, Cleveland Clinic
contact email	zhuj6@ccf.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD068796

PRIDE project URI

• PRIDE
  1. PXD068796
     1. Label: PRIDE project
     2. Name: SARS-CoV-2 Nsp3 interactome in Caco-2 cells during authentic infection