PXD068787

PXD068787 is an original dataset announced via ProteomeXchange.

Title	Direct Coupling of Nod Factor Signaling to Vesicular Trafficking Initiates Legume Nodulation
Description	Legume–rhizobium symbiosis requires coordinated receptor signaling and membrane trafficking to initiate infection thread formation in root hairs. Here, we identify the soybean Qa-SNARE GmSYNTAXIN111a (GmSYP111a), a close paralog of the cytokinesis-associated protein KNOLLE, as a critical regulator of symbiotic infection. Kinase-client assays and in vivo immunoprecipitation–mass spectrometry showed that GmSYP111a is phosphorylated by the receptor kinase GmSymRKβ at Ser-8 and Ser-128. BiFC, co-immunoprecipitation, and kinase assays validated the interaction and phosphorylation. Nod factor perception promoted clathrin-mediated endocytosis of the GmSymRKβ–GmSYP111a complex and its relocalization to intracellular vesicles. Structural modeling and interaction assays suggest that dual phosphorylation exposes an endocytic motif that recruits the TPLATE adaptor; accordingly, non-phosphorylatable mutations impaired internalization, whereas phosphomimetic substitutions induced endocytosis without rhizobial stimulation. GmSYP111a also interacted with VAMP72, linking endocytic recruitment to vesicle fusion. Genetic analyses in soybean and Lotus japonicus established a conserved requirement for GmSYP111a in nodule initiation. These findings define a phosphorylation-dependent SNARE switch that couples Nod factor signaling to membrane trafficking during legume nodulation.
HostingRepository	PRIDE
AnnounceDate	2026-07-10
AnnouncementXML	Submission_2026-07-10_01:40:05.396.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriel Lemes Jorge
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; scientific name: Glycine max; NCBI TaxID: NEWT:3847;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 14:55:13	ID requested
⏵ 1	2026-07-10 01:40:05	announced

10.1038/S41467-026-75535-2;

submitter keyword: Legume–rhizobium symbiosis, Clathrin-mediated endocytosis,kinase client assay, Infection thread initiation, Soybean nodulation, Phosphorylation, SNARE complex regulation

Gary Stacey
contact affiliation	C.S. Bond Life Science Center, Division of Plant Sciences and Technology, University of Missouri, Columbia, MO, United States
contact email	staceyg@missouri.edu
lab head
Gabriel Lemes Jorge
contact affiliation	University of Missouri
contact email	glj2d5@umsystem.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068787
     1. Label: PRIDE project
     2. Name: Direct Coupling of Nod Factor Signaling to Vesicular Trafficking Initiates Legume Nodulation