PXD068767

PXD068767 is an original dataset announced via ProteomeXchange.

Title	MASP-II for Mapping the Whole-tissue Spatial Proteome and the Intra-brain Distribution of Monoclonal Antibodies
Description	Spatial proteomics at whole-tissue level provides critical insights into region-specific biological regulations but remains challenging. Previously, we introduced the Micro-scaffold Assisted Spatial Proteomics (MASP) technique, permitting whole-tissue mapping. While promising, this prototype required substantial improvements in spatial resolution, practicality, and throughput for practical application. Here we present a next-generation MASP technique (MASP-II) featuring i) a new design of hexagonal-micro-wells fabricated with optimized Projection Micro-Stereolithography (PµSL) 3D-printing, achieving high spatial resolution, sampling robustness and mechanical strength for reproducibly compartmentalizing even tough tissues; ii) enhanced throughput/effectiveness in sample preparation and LC-MS analysis with high quantitative quality. Using MASP-II, we generated in-depth, whole-tissue maps for >6,000 proteins in mouse brains, with thoroughly validated mapping accuracy. The improved resolution of MASP-II revealed critical details that were previously absent in maps by its prototype, enabling precise depiction of protein distribution heterogeneity. This technique enabled the discovery of many novel regionally enriched proteins across brain structures. Applying MASP-II to study the intra-brain distribution of intracerebroventricularly-dosed antibody drugs and related proteins revealed novel insights into antibody distribution mechanisms and local effects induced by drug treatment.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-24_09:12:41.148.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Ma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 06:54:06	ID requested
⏵ 1	2026-08-24 09:12:41	announced

Dataset with its publication pending

submitter keyword: MASP, whole tissue mapping, accurate protein distribution, in-depth spatial proteomics, mAb intra-brain distribution

Jun Qu
contact affiliation	Department of Pharmaceutical Sciences, University at Buffalo, US
contact email	junqu@buffalo.edu
lab head
Min Ma
contact affiliation	University at Buffalo
contact email	minma@buffalo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD068767

PRIDE project URI

• PRIDE
  1. PXD068767
     1. Label: PRIDE project
     2. Name: MASP-II for Mapping the Whole-tissue Spatial Proteome and the Intra-brain Distribution of Monoclonal Antibodies