PXD068765

PXD068765 is an original dataset announced via ProteomeXchange.

Title	In-vitro Co-immunoprecipitation Mass Spectrometry of LUH and AP1 from Arabidopsis
Description	This dataset contains quantitative co-immunoprecipitation mass spectrometry (Co-IP-MS) data generated to investigate direct interactions between the Arabidopsis co-regulator LUH and the MADS-domain transcription factor AP1. LUH wild type and domain variants (LUH_W, LUH_QW, LUH_LQ) were co-expressed with AP1 or empty vector controls, and proteins were immunoprecipitated using AP1-specific antibodies. Input and eluate fractions were analyzed by LC-MS/MS and quantified using MaxQuant. The results show that LUH interacts directly with AP1 via its C-terminal domains, supporting a distinct mode of co-regulator-MADS TF association.
HostingRepository	PRIDE
AnnounceDate	2025-11-25
AnnouncementXML	Submission_2025-11-25_03:29:29.114.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cezary Smaczniak
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; scientific name: Triticum aestivum (Wheat); NCBI TaxID: NEWT:4565;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 06:17:35	ID requested
⏵ 1	2025-11-25 03:29:30	announced

10.1111/tpj.70585;

Vega-Le, ó, n R, Muino JM, Zhu T, Gluza JA, Pimentel M, Rehbein K, Steiner S, Rezzolla E, Golz JF, Chen D, Kaufmann K, Smaczniak C, Rewiring of LEUNIG_HOMOLOG interaction networks marks regulatory shifts from meristem to organ growth in Arabidopsis flowers. Plant J, 124(4):e70585(2025) [pubmed]

submitter keyword: Arabidopsis, AP1,LUH, Co-IP-MS

Kerstin Kaufmann
contact affiliation	Department for Plant Cell and Molecular Biology, Institute for Biology, Humboldt-Universität zu Berlin
contact email	kerstin.kaufmann@hu-berlin.de
lab head
Cezary Smaczniak
contact affiliation	Department for Plant Cell and Molecular Biology, Institute for Biology, Humboldt-Universität zu Berlin
contact email	cezary.smaczniak@hu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD068765

PRIDE project URI

• PRIDE
  1. PXD068765
     1. Label: PRIDE project
     2. Name: In-vitro Co-immunoprecipitation Mass Spectrometry of LUH and AP1 from Arabidopsis