PXD068764

PXD068764 is an original dataset announced via ProteomeXchange.

Title	Negative feedback regulation of the hemi-arrestin MAPK scaffold Sms1 prevents untimely mating
Description	Mitogen-activated protein kinases (MAPK) are ancestral kinases that form essential signalling cascades. However, scaffolds that recruit kinases to subcellular locations and promote signal transduction have only been described in few species. Notably, no scaffold was thought necessary for the MAPK cascade promoting sexual differentiation in fission yeast. Here, we identify the hemi-arrestin protein Sms1 as a structurally novel scaffold of this MAPK cascade. Interactions with PIP₂ and the pheromone receptor–coupled Gα subunit target Sms1 to plasma membrane patches, where it assembles the active cascade by binding each MAP kinase. These interactions are essential for signal transduction and local signal interpretation for polarised growth. Phosphorylation, including by the MAPK itself, antagonizes Sms1 membrane translocation, establishing a negative feedback that underlies polarity patch turnover and prevents untimely mating attempts. Thus, Sms1 is a MAPK scaffold with canonical functions despite its distinct structural fold, highlighting convergent evolution of MAPK scaffolds across eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2026-02-19
AnnouncementXML	Submission_2026-02-18_22:40:44.529.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie Martin
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: NEWT:4896;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 06:07:10	ID requested
⏵ 1	2026-02-18 22:40:45	announced

10.1101/2025.09.24.678297;

submitter keyword: convergent evolution, yeast Schizosaccharomyces pombe,Mitogen-activated protein kinase (MAPK), negative feedback, MAPK scaffold

Sophie Martin
contact affiliation	Dept. of Molecular and Cellular Biology University of Geneva Quai Ernest-Ansermet 30 1211 Geneva 4
contact email	Sophie.Martin@unige.ch
lab head
Sophie Martin
contact affiliation	University of Geneva
contact email	sophie.martin@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068764
     1. Label: PRIDE project
     2. Name: Negative feedback regulation of the hemi-arrestin MAPK scaffold Sms1 prevents untimely mating