⮝ Full datasets listing
PXD068755
PXD068755 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of PHACTR1 overexpressing and knockdown HT1080 stable cell lines |
| Description | The PHACTR1 gene to a range of vascular and neurological diseases, underscoring its pivotal role in biology. To investigate the role of PHACTR1 in cells, we used lentiviral transfection of HT1080 cells with plasmids to overexpress (O) or knockdown (K) the PHACTR1 gene or a scrambled control plasmid (S) and created stable cell lines. As part of a larger multi-omics study, we performed untargeted on cell lysates (n=6 per condition). Peptide extracts were fractionated into 8, and analysed by LC-MS/MS using a C18AQ column on an Ultimate nanoRSLC UPLC coupled to a Orbitrap Fusion Lumos. Mass spectrometry raw data files were searched using MaxQuant (v2.1.3.0) against the Uniprot human canonical proteome (downloaded April 2023). |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-17 |
| AnnouncementXML | Submission_2025-12-17_00:13:43.231.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kathryn Wolhuter |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl; Carboxymethyl; methionine oxidation with neutral loss of 80 Da; methionine oxidation with neutral loss of 64 Da |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-23 21:36:06 | ID requested | |
| ⏵ 1 | 2025-12-17 00:13:43 | announced |
Publication List
| Kathryn Wolhuter, Lijiang Ma, Nicole S Bryce, Osvaldo Contreras, Natalie Mellett, Ling Zhong, Chris Thekkedam, Corey Giles, Richard P Harvey, Thomas Hennessy, Chris Fouracre, David Bradley, Peter J Meikle, Johan L M Björkegren, Jason C Kovacic. Multi-omic Analysis of PHACTR1 Signaling Networks in Human Cells. Communications Biology (accepted). |
Keyword List
| submitter keyword: PHACTR1, HT1080, global proteomics, LC-MS/MS, MaxQuant, DatasetType:Proteomics |
Contact List
| Jason C Kovacic | |
|---|---|
| contact affiliation | Victor Chang Cardiac Research Institute |
| contact email | J.Kovacic@victorchang.edu.au |
| lab head | |
| Kathryn Wolhuter | |
| contact affiliation | Victor Chang Cardiac Research Institute |
| contact email | k.wolhuter@victorchang.edu.au |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099266/ |
