PXD068739

PXD068739 is an original dataset announced via ProteomeXchange.

Title	Supplementary Dataset S7. ADP-ribosylated peptides identified in two Af1521 Macro pulldown experiments from HopU1-Flag vs. Col-0 samples by the ‘triggering’ method.
Description	During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein HopU1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to compare affinity enrichment of ADP-ribosylated Arabidopsis thaliana proteins using the enhanced Af1521 Macro domain versus a G42E mutant variant as control.
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-16_23:02:37.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lennart Wirthmueller
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-23 07:07:39	ID requested
⏵ 1	2025-11-16 23:02:37	announced

10.1002/PLD3.70115;

submitter keyword: HopU1, LC-MSMS, Arabidopsis thaliana, affinity purification,plant, Pseudomonas syringae, ADP-ribosylation, plant pathogen, type 3 effector

Lennart Wirthmueller
contact affiliation	Leibniz Institute of Plant Biochemistry, Department Biochemistry of Plant Interactions, Weinberg 3, 06120 Halle (Saale), Germany
contact email	lennart.wirthmueller@ipb-halle.de
lab head
Lennart Wirthmueller
contact affiliation	Leibniz Institute of Plant Biochemistry Dept. Biochemistry of Plant Interactions Weinberg 3 06120 Halle (Saale)
contact email	lennart.wirthmueller@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068739
     1. Label: PRIDE project
     2. Name: Supplementary Dataset S7. ADP-ribosylated peptides identified in two Af1521 Macro pulldown experiments from HopU1-Flag vs. Col-0 samples by the ‘triggering’ method.