PXD068731

PXD068731 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics of Acute Myeloid Leukemia (AML) Blast cells after Therapy-Induced Senescence (TIS) with Cytarabine.
Description	Resistance or relapse upon chemotherapy are major determinants of treatment failure in acute myeloid leukemia (AML). Therapy-induced senescence (TIS) is a potential outcome of chemotherapy, but its immunological consequences in AML remain unclear. We show that ex-vivo chemotherapy induces senescence in a subset of therapy-naïve AML samples. TIS is marked by elevated interferon signaling, upregulation of HLA class I/II molecules, and increased presentation of leukemia and senescence-associated peptides, conferring AML blasts antigen-presenting cell-like features. Patient-derived leukemic blasts purified from AML samples were either treated with cytarabine (AraC) or left untreated. Further, samples that exhibited a significant increase in fluorescent SA-β-Gal upon treatment were classified as Senescence High (Sen High), whereas others that showed minimal change were classified as Senescence Low (Sen Low). We employed our in-house microfluidic platform, PeptiCHIP for immunoaffinity purification followed by Ligandomics LC/MS analysis, to identify HLA class I-bound peptides in Patient-derived leukemic blasts, seeking to compare the immunopeptidome repertoire of Sen Low and Sen High samples before and after cytarabine treatment. Sen High exhibited a greater number of eluted peptides compared to Sen Low samples already at baseline. This number was further increased following chemotherapy in all Sen High samples analyzed. Conversely, the number of peptides retrieved in Sen Low samples remained low regardless of chemotherapy treatment.
HostingRepository	PRIDE
AnnounceDate	2026-08-20
AnnouncementXML	Submission_2026-08-19_22:38:29.059.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Branca
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-23 03:04:44	ID requested
⏵ 1	2026-08-19 22:38:29	announced

Dataset with its publication pending

submitter keyword: Immunopeptidomics, Therapy-Induced Senescence, TIS, HLA, Ligandome, MHC, cytarabine, Ligandomics, Immunopeptidome, TimsTOF SCP, Acute Myeloid Leukemia, AML

Janne Lehtiö
contact affiliation	Dept. Oncology-Pathology, Scilifelab, Karolinska Institutet, Sweden
contact email	janne.lehtio@ki.se
lab head
Rui Branca
contact affiliation	Clinical Proteomics Unit, Dep. of Oncology-Pathology
contact email	rui.mamede-branca@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068731
     1. Label: PRIDE project
     2. Name: Immunopeptidomics of Acute Myeloid Leukemia (AML) Blast cells after Therapy-Induced Senescence (TIS) with Cytarabine.