PXD068715

PXD068715 is an original dataset announced via ProteomeXchange.

Title	Exploring the phosphor-proteome in B16 cells wild type cells or TAK1 CRISPR KO cells upon exposure to combined TNF and Interferon Gamma stimulation.
Description	Global phosphoproteomic profiling of wild-type and TAK1-deficient B16F10 cells treated with TNF (10ng/ml) and IFNγ (1ng/ml) for one hour. The analysis reveals that TAK1 loss selectively disrupts phosphorylation of NF-κB and MAPK pathway components, including RELA, RIPK2, and MAP3K8, while STAT1-associated phosphorylation events remain intact, indicating that IFNγ–STAT1 signaling is TAK1 independent. Stratification of phosphosites highlights TAK1-sensitive sites, which are induced in wild-type but lost in TAK1 knockout cells, and TAK1-independent sites, which remain unaffected by TAK1 loss. Notably, phosphorylation of RIPK1 was altered: the inhibitory S25 site was reduced in TAK1-deficient cells, while a cluster of sites spanning Y309–S415 was more prominently phosphorylated, consistent with enhanced pro-death signaling. Together, these data show that TAK1 sustains NF-κB–driven survival signaling while restraining RIPK1 activation, thereby acting as a molecular switch that biases TNF and IFNγ signaling toward cytoprotection; its loss rewires cytokine signaling toward apoptosis.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_21:43:20.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chloe Gerak
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-22 19:45:53	ID requested
⏵ 1	2025-11-09 21:43:21	announced

Dataset with its publication pending

submitter keyword: TNF,B16 cells, Interferon Gamma, mouse

Stephin Vervoort
contact affiliation	Lab head, WEHI, Melbourne, Australia
contact email	vervoort.s@wehi.edu.au
lab head
Chloe Gerak
contact affiliation	Walter and Eliza Hall Institute
contact email	gerak.c@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD068715

PRIDE project URI

• PRIDE
  1. PXD068715
     1. Label: PRIDE project
     2. Name: Exploring the phosphor-proteome in B16 cells wild type cells or TAK1 CRISPR KO cells upon exposure to combined TNF and Interferon Gamma stimulation.